Activation of human CD8-positive T cells via the CD8/HLA class I complex

Yuri Bushkin, Sandra Demaria, Nahid Mohagheghpour, Junming Le

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Cross-linking of CD8 and HLA class I molecules with appropriate monoclonal antibodies (mAb) and goat anti-mouse Ig (GaMIg) antibody resulted in a marked proliferation of resting human CD8 cells in the presence of interleukin-2 (IL-2). These cells also expressed IL-2 receptor (IL-2R), transferrin receptor, HLA-DR and -DQ antigens. Activation of the cross-linked CD8 cells is apparently independent of accessory monocytes. Various anti-CD8 and anti-HLA class I mAb recognizing nonpolymorphic antigenic determinants were examined for the efficacy of activating CD8 cells. Among mAb specific for HLA class I molecules, PA2.6, MB40.5, BB7.7, A1.4, and W6/32 mAb markedly stimulated the proliferation of cross-linked CD8 cells, whereas BBM.1, Q1/28, and HC10 mAb were found inactive. Footprinting analysis of HLA class I molecules suggested that the activity of these anti-HLA class I mAb appeared to be related to the corresponding peptides they protect from enzymatic digestion. In contrast to the anti-HLA class I mAb, all anti-CD8 mAb examined (C8, OKT8A, and anti-Leu-2a) induced the proliferation of CD8-HLA class I cross-linked cells with similar efficacy. These results suggest that physical interaction between CD8 and at least one specific region of HLA class I molecules can trigger the activation of resting human CD8 cells.

Original languageEnglish (US)
Pages (from-to)185-195
Number of pages11
JournalCellular Immunology
Volume126
Issue number1
DOIs
StatePublished - Jan 1 1990

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Monoclonal Antibodies
T-Lymphocytes
HLA-DQ Antigens
Transferrin Receptors
Interleukin-2 Receptors
HLA-DR Antigens
Goats
Interleukin-2
Epitopes
Monocytes
Digestion
Peptides
Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Bushkin, Yuri ; Demaria, Sandra ; Mohagheghpour, Nahid ; Le, Junming. / Activation of human CD8-positive T cells via the CD8/HLA class I complex. In: Cellular Immunology. 1990 ; Vol. 126, No. 1. pp. 185-195.
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Activation of human CD8-positive T cells via the CD8/HLA class I complex. / Bushkin, Yuri; Demaria, Sandra; Mohagheghpour, Nahid; Le, Junming.

In: Cellular Immunology, Vol. 126, No. 1, 01.01.1990, p. 185-195.

Research output: Contribution to journalArticle

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