Active center rearrangement in RNA polymerase initiation complex

Arkady Muetaev, Mikhail Kashlev, Evgeny Zaychikov, Mikhail Grachev, Alex Goldfarb

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32 Scopus citations


His1237 in the β subunit of Escherichia coli RNA polymerase marks the '5' face' of the active center since it can be cross-linked to the γ- phosphate of the priming substrate. It is demonstrated that RNA chains up to 9 nucleotides in length can be synthesized using His1237-cross-linked nucleotide as a primer. Thus, a substantial mass of RNA can be accommodated in the active center between His1237 and the site of catalysis that remains juxtaposed to the growing 3' end. The apparent 'filling' of the active center with RNA precedes promoter clearance and suggests a mechanism of coupling between catalysis and saltatory translocation of RNA polymerase.

Original languageEnglish (US)
Pages (from-to)19185-19187
Number of pages3
JournalJournal of Biological Chemistry
Issue number26
StatePublished - Jan 1 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Muetaev, A., Kashlev, M., Zaychikov, E., Grachev, M., & Goldfarb, A. (1993). Active center rearrangement in RNA polymerase initiation complex. Journal of Biological Chemistry, 268(26), 19185-19187.