Statement of Purpose: Reversible addition-fragmentation chain transfer (RAFT) polymerization enables precise control over molecular weight and polydispersity and allows for investigation of complex polymer architectures with bioactive functionality (Yeow, Chapman et al. 2017). Traditionally, this oxygen-intolerant polymer chemistry is done in batch processes that are technically challenging and time consuming to complete. Recently, we have shown that oxygen-tolerant porphyrin-catalyzed photoinduced electron transfer RAFT (PET-RAFT) polymerization enables combinatorial preparation of bioactive polymers in 96 or 384 well plates (Gormley, Yeow et al. 2018).1 To further expand on this unique capability and make polymer chemistry accessible to the non-expert, we have programmed a Hamilton Microlab STARlet liquid handling robot to perform high throughput polymer chemistry and bioconjugation based on a user-friendly graphical user interface (GUI). This automation allows for the reliable and simple creation of customizable polymers for a variety of biological applications.