Addition of 2-deoxyglucose enhances growth inhibition but reverses acidification in colon cancer cells treated with phenformin

Michael A. Lea, Jerel Chacko, Sandhya Bolikal, Ji Y. Hong, Ryan Chung, Andres Ortega, Charles DesBordes

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

A report that effects of butyrate on some cells may be mediated by activation of AMP-activated protein kinase (AMPK) prompted this study which examines if other AMPK activators can induce differentiation and inhibit proliferation of colon cancer cells in a manner similar to butyrate. Using induction of alkaline phosphatase as a marker, it was observed that compound C, an AMPK inhibitor, is able to reduce the differentiating effect of butyrate on SW1116 and Caco-2 colon cancer cells. Metformin was observed to be less effective than butyrate in the induction of alkaline phosphatase but was more effective as a growth inhibitor. Phenformin was found to be a more potent growth inhibitor than metformin and both compounds cause acidification of the medium when incubated with colon cancer cells. Combined incubation of 2-deoxyglucose with either of the biguanides prevented the acidification of the medium but enhanced the growth inhibitory effects.

Original languageEnglish (US)
Pages (from-to)421-426
Number of pages6
JournalAnticancer Research
Volume31
Issue number2
StatePublished - Feb 1 2011

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Keywords

  • 2-deoxyglucose
  • Acidification
  • Colon cancer
  • Metformin
  • Phenformin

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