Advanced stoichiometric analysis of metabolic networks of mammalian Systems

Mehmet A. Orman, Francois Berthiaume, Ioannis P. Androulakis, Marianthi G. Ierapetritou

Research output: Contribution to journalReview articlepeer-review

24 Scopus citations


Metabolic engineering tools have been widely applied to living organisms to gain a comprehensive understanding about cellular networks and to improve cellular properties. Metabolic flux analysis (MFA), flux balance analysis (FBA), and metabolic pathway analysis (MPA) are among the most popular tools in stoichiometric network analysis. Although application of these tools into well-known microbial systems is extensive in the literature, various barriers prevent them from being utilized in mammalian cells. Limited experimental data, complex regulatory mechanisms, and the requirement of more complex nutrient media are some major obstacles in mammalian cell systems. However, mammalian cells have been used to produce therapeutic proteins, to characterize disease states or related abnormal metabolic conditions, and to analyze the toxicological effects of some medicinally important drugs. Therefore, there is a growing need for extending metabolic engineering principles to mammalian cells in order to understand their underlying metabolic functions. In this review article, advanced metabolic engineering tools developed for stoichiometric analysis including MFA, FBA, and MPA are described. Applications of these tools in mammalian cells are discussed in detail, and the challenges and opportunities are highlighted.

Original languageEnglish (US)
Pages (from-to)511-534
Number of pages24
JournalCritical reviews in biomedical engineering
Issue number6
StatePublished - 2011

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering


  • Flux balance analysis
  • Mammalian cells
  • Metabolic flux analysis
  • Metabolic pathway analysis


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