Aggregatibacter actinomycetemcomitans - Induced bone loss and antibody response in three rat strains

Helen Schreiner, Kenneth Markowitz, Manjula Miryalkar, Danielle Moore, Scott Diehl, Daniel H. Fine

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Background: The aimof this study is to compare the colonization, immunoglobulin (Ig)G response, and alveolar bone loss in Aggregatibacter actinomycetemcomitans (Aa)-inoculated Fawn Hooded Hypertensive (FHH), Dahl Salt-Sensitive (DSS), and Brown Norway (BN) rats. Methods: Each rat strain was divided into wild-type Aainoculated and non-inoculated control groups. Blood taken at 12 weeks after inoculation was assessed for Aa-specific IgG antibodies by an enzyme-linked immunosorbent assay. Colonization was assessed 12 weeks postinoculation. Bone loss was estimated bymeasuring the distance from the cemento-enamel junction (CEJ) to the alveolar bone crest (ABC) at 20 molar sites. Colonization and antibody levels were compared by using the Student t test. Diseased ratsweredefinedas having twosites per quadrant with CEJ-ABC distances that were significantly greater than the control CEJ-ABC distances. Results: The Aa colonization of FHH rats was significantly higher than in other strains (P <0.05). The Aa-specific IgG levels in theDSS Aa-inoculated groupwere significantly higher than in its control group (P <0.05). Only FHH rats showed Aa diseaseassociated bone loss (P = 0.0021). Conclusions: Aa colonized and caused more disease in FHH rats than in the other rat strains. The rat strains each responded differently to the same Aa strain.

Original languageEnglish (US)
Pages (from-to)142-150
Number of pages9
JournalJournal of periodontology
Volume82
Issue number1
DOIs
StatePublished - Jan 1 2011

All Science Journal Classification (ASJC) codes

  • Periodontics

Keywords

  • Alveolar bone loss
  • Animals
  • Disease models
  • Host-pathogen interactions
  • Periodontitis

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