Allelic variation linked to adenine phosphoribosyltransferase locus in mouse teratocarcinoma cell line and feral-derived mouse strains

Mitchell S. Turker, Peter J. Stambrook, Jay Tischfield, Annette C. Smith, George M. Martin

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Southern blot analysis reveals two distinct adenine phosphoribosyltransferase (APRT)alleles in the P-19 mouse teratocarcinoma cell line. One allele is identical to that observed in common laboratory mouse strains (Mus musculus domesticus). The restriction enzyme site variations between the two alleles occur in sequences located both upstream and downstream of the APRT gene, but not within it. Although the P-19 cell line was established from a C3H strain embryo (Mus musculus domesticus), a sixth generation ancestor of this embryo was a feral mouse (Mus musculus musculus). The restriction pattern of the variant APRT allele in P-19 is identical to that of a feral-derived Mus musculus musculus animal, establishing the origin of this allele in the P-19 cell line. A third, distinct APRT allele was found in a Mus spretus feral-derived mouse. Exploiting the differences between the two APRT alleles in the P-19 cell line, we have demonstrated their sequential loss in APRT-deficient clones.

Original languageEnglish (US)
Pages (from-to)159-166
Number of pages8
JournalSomatic Cell and Molecular Genetics
Volume15
Issue number2
DOIs
StatePublished - Mar 1 1989
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

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