Alterations in fingerprints of polychlorinated biphenyls in benthic biota at the Portland Harbor Superfund Site (Oregon, USA) suggest metabolism

Lisa Rodenburg, Damon A. Delistraty

Research output: Contribution to journalArticle

Abstract

In order to understand the sources and fate of polychlorinated biphenyls (PCBs) in several species of benthic biota, including clams (Corbicula fluminea), oligochaetes (Lumbriculus variegatus), and mussels (Margaritifera falcata and Anodonta nuttalliana) at the Portland Harbor Superfund Site (PHSS), their congener fingerprints were examined. First, diagnostic ratios of congeners known to be metabolizable vs. recalcitrant in the cytochrome P450 (CYP) pathway were significantly lower in biota than in its co-located sediment, indicating metabolism may have occurred. Next, the congener patterns were analyzed using Positive Matrix Factorization (PMF). The dominant fingerprint (by mass) in benthic biota is related to Aroclor 1260 but displays differences in the fingerprint that are consistent with weathering via absorption, distribution, metabolism, and excretion (ADME). This fingerprint is similar to one isolated from PCBs in fish from Washington State, indicative of common metabolic pathways and consistent with CYP metabolism. When metabolism is taken into account, the spatial distribution of the PMF-isolated PCB fingerprints in biota matches well with those from co-located sediment samples, suggesting that the same mix of sources at one location partitions into biota and sediment. In accordance to their higher hydrophobicity, higher molecular weight (MW) PCB formulations were proportionately more abundant in biota than in sediment, although low MW PCBs (e.g., PCBs 4 and 11) do bioaccumulate in benthic organisms and should not be ignored in risk assessment efforts. Finally, fingerprinting suggests potential reasons why lab-based and field-based biota-sediment accumulation factors (BSAFs) differ substantially for bivalves.

Original languageEnglish (US)
Pages (from-to)74-82
Number of pages9
JournalChemosphere
Volume223
DOIs
StatePublished - May 1 2019

Fingerprint

Biota
Superfund
Polychlorinated Biphenyls
Dermatoglyphics
Ports and harbors
Polychlorinated biphenyls
Metabolism
biota
PCB
harbor
metabolism
Sediments
sediment
Bivalvia
Factorization
Cytochrome P-450 Enzyme System
cytochrome
Corbicula
Anodonta
Molecular Weight

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

Keywords

  • Bioaccumulation
  • Biota
  • Metabolism
  • PCBs
  • Source apportionment

Cite this

@article{7f3ea7cb307643ef9c0d7666d734dc66,
title = "Alterations in fingerprints of polychlorinated biphenyls in benthic biota at the Portland Harbor Superfund Site (Oregon, USA) suggest metabolism",
abstract = "In order to understand the sources and fate of polychlorinated biphenyls (PCBs) in several species of benthic biota, including clams (Corbicula fluminea), oligochaetes (Lumbriculus variegatus), and mussels (Margaritifera falcata and Anodonta nuttalliana) at the Portland Harbor Superfund Site (PHSS), their congener fingerprints were examined. First, diagnostic ratios of congeners known to be metabolizable vs. recalcitrant in the cytochrome P450 (CYP) pathway were significantly lower in biota than in its co-located sediment, indicating metabolism may have occurred. Next, the congener patterns were analyzed using Positive Matrix Factorization (PMF). The dominant fingerprint (by mass) in benthic biota is related to Aroclor 1260 but displays differences in the fingerprint that are consistent with weathering via absorption, distribution, metabolism, and excretion (ADME). This fingerprint is similar to one isolated from PCBs in fish from Washington State, indicative of common metabolic pathways and consistent with CYP metabolism. When metabolism is taken into account, the spatial distribution of the PMF-isolated PCB fingerprints in biota matches well with those from co-located sediment samples, suggesting that the same mix of sources at one location partitions into biota and sediment. In accordance to their higher hydrophobicity, higher molecular weight (MW) PCB formulations were proportionately more abundant in biota than in sediment, although low MW PCBs (e.g., PCBs 4 and 11) do bioaccumulate in benthic organisms and should not be ignored in risk assessment efforts. Finally, fingerprinting suggests potential reasons why lab-based and field-based biota-sediment accumulation factors (BSAFs) differ substantially for bivalves.",
keywords = "Bioaccumulation, Biota, Metabolism, PCBs, Source apportionment",
author = "Lisa Rodenburg and Delistraty, {Damon A.}",
year = "2019",
month = "5",
day = "1",
doi = "10.1016/j.chemosphere.2019.02.039",
language = "English (US)",
volume = "223",
pages = "74--82",
journal = "Chemosphere",
issn = "0045-6535",
publisher = "Elsevier Limited",

}

Alterations in fingerprints of polychlorinated biphenyls in benthic biota at the Portland Harbor Superfund Site (Oregon, USA) suggest metabolism. / Rodenburg, Lisa; Delistraty, Damon A.

In: Chemosphere, Vol. 223, 01.05.2019, p. 74-82.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Alterations in fingerprints of polychlorinated biphenyls in benthic biota at the Portland Harbor Superfund Site (Oregon, USA) suggest metabolism

AU - Rodenburg, Lisa

AU - Delistraty, Damon A.

PY - 2019/5/1

Y1 - 2019/5/1

N2 - In order to understand the sources and fate of polychlorinated biphenyls (PCBs) in several species of benthic biota, including clams (Corbicula fluminea), oligochaetes (Lumbriculus variegatus), and mussels (Margaritifera falcata and Anodonta nuttalliana) at the Portland Harbor Superfund Site (PHSS), their congener fingerprints were examined. First, diagnostic ratios of congeners known to be metabolizable vs. recalcitrant in the cytochrome P450 (CYP) pathway were significantly lower in biota than in its co-located sediment, indicating metabolism may have occurred. Next, the congener patterns were analyzed using Positive Matrix Factorization (PMF). The dominant fingerprint (by mass) in benthic biota is related to Aroclor 1260 but displays differences in the fingerprint that are consistent with weathering via absorption, distribution, metabolism, and excretion (ADME). This fingerprint is similar to one isolated from PCBs in fish from Washington State, indicative of common metabolic pathways and consistent with CYP metabolism. When metabolism is taken into account, the spatial distribution of the PMF-isolated PCB fingerprints in biota matches well with those from co-located sediment samples, suggesting that the same mix of sources at one location partitions into biota and sediment. In accordance to their higher hydrophobicity, higher molecular weight (MW) PCB formulations were proportionately more abundant in biota than in sediment, although low MW PCBs (e.g., PCBs 4 and 11) do bioaccumulate in benthic organisms and should not be ignored in risk assessment efforts. Finally, fingerprinting suggests potential reasons why lab-based and field-based biota-sediment accumulation factors (BSAFs) differ substantially for bivalves.

AB - In order to understand the sources and fate of polychlorinated biphenyls (PCBs) in several species of benthic biota, including clams (Corbicula fluminea), oligochaetes (Lumbriculus variegatus), and mussels (Margaritifera falcata and Anodonta nuttalliana) at the Portland Harbor Superfund Site (PHSS), their congener fingerprints were examined. First, diagnostic ratios of congeners known to be metabolizable vs. recalcitrant in the cytochrome P450 (CYP) pathway were significantly lower in biota than in its co-located sediment, indicating metabolism may have occurred. Next, the congener patterns were analyzed using Positive Matrix Factorization (PMF). The dominant fingerprint (by mass) in benthic biota is related to Aroclor 1260 but displays differences in the fingerprint that are consistent with weathering via absorption, distribution, metabolism, and excretion (ADME). This fingerprint is similar to one isolated from PCBs in fish from Washington State, indicative of common metabolic pathways and consistent with CYP metabolism. When metabolism is taken into account, the spatial distribution of the PMF-isolated PCB fingerprints in biota matches well with those from co-located sediment samples, suggesting that the same mix of sources at one location partitions into biota and sediment. In accordance to their higher hydrophobicity, higher molecular weight (MW) PCB formulations were proportionately more abundant in biota than in sediment, although low MW PCBs (e.g., PCBs 4 and 11) do bioaccumulate in benthic organisms and should not be ignored in risk assessment efforts. Finally, fingerprinting suggests potential reasons why lab-based and field-based biota-sediment accumulation factors (BSAFs) differ substantially for bivalves.

KW - Bioaccumulation

KW - Biota

KW - Metabolism

KW - PCBs

KW - Source apportionment

UR - http://www.scopus.com/inward/record.url?scp=85061649478&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85061649478&partnerID=8YFLogxK

U2 - 10.1016/j.chemosphere.2019.02.039

DO - 10.1016/j.chemosphere.2019.02.039

M3 - Article

VL - 223

SP - 74

EP - 82

JO - Chemosphere

JF - Chemosphere

SN - 0045-6535

ER -