Aminoglycoside-3″-adenyltransferase confers resistance to spectinomycin and streptomycin in Nicotiana tabacum

Zora Svab; Elisabeth C. Harper; Jonathan D.G. Jones; Pal Maliga

doi:10.1007/BF00018560

Aminoglycoside-3″-adenyltransferase confers resistance to spectinomycin and streptomycin in Nicotiana tabacum

Zora Svab, Elisabeth C. Harper, Jonathan D.G. Jones, Pal Maliga

Research output: Contribution to journal › Article › peer-review

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Abstract

The bacterial gene aad A encodes the enzyme aminoglycoside-3″-adenyltransferase that confers resistance to spectinomycin and streptomycin in Escherichia coli. Chimeric genes have been constructed for expression in plants, and were introduced into Nicotiana tabacum by Agrobacterium binary transformation vectors. Spectinomycin or streptomycin in selective concentrations prevent greening of N. tabacum calli. Transgenic clones, however, formed green calli on selective media containing spectinomycin, streptomycin, or both drugs. Resistance was inherited as a dominant Mendelian trait in the seed progeny. Resistance conferred by the chimeric aad A gene can be used as a color marker similar to the resistance conferred by the streptomycin phosphotransferase gene to streptomycin.

Original language	English (US)
Pages (from-to)	197-205
Number of pages	9
Journal	Plant Molecular Biology
Volume	14
Issue number	2
DOIs	https://doi.org/10.1007/BF00018560
State	Published - Feb 1990
Externally published	Yes

All Science Journal Classification (ASJC) codes

Agronomy and Crop Science
Genetics
Plant Science

Keywords

Aminoglycoside-3″-adenyltransferase
Nicotiana tabacum
chimeric gene
spectinomycin resistance
streptomycin resistance
transgenic plant

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10.1007/BF00018560

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title = "Aminoglycoside-3″-adenyltransferase confers resistance to spectinomycin and streptomycin in Nicotiana tabacum",

abstract = "The bacterial gene aad A encodes the enzyme aminoglycoside-3″-adenyltransferase that confers resistance to spectinomycin and streptomycin in Escherichia coli. Chimeric genes have been constructed for expression in plants, and were introduced into Nicotiana tabacum by Agrobacterium binary transformation vectors. Spectinomycin or streptomycin in selective concentrations prevent greening of N. tabacum calli. Transgenic clones, however, formed green calli on selective media containing spectinomycin, streptomycin, or both drugs. Resistance was inherited as a dominant Mendelian trait in the seed progeny. Resistance conferred by the chimeric aad A gene can be used as a color marker similar to the resistance conferred by the streptomycin phosphotransferase gene to streptomycin.",

keywords = "Aminoglycoside-3″-adenyltransferase, Nicotiana tabacum, chimeric gene, spectinomycin resistance, streptomycin resistance, transgenic plant",

author = "Zora Svab and Harper, {Elisabeth C.} and Jones, {Jonathan D.G.} and Pal Maliga",

year = "1990",

month = feb,

doi = "10.1007/BF00018560",

language = "English (US)",

volume = "14",

pages = "197--205",

journal = "Plant Molecular Biology",

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publisher = "Springer Netherlands",

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AU - Svab, Zora

AU - Harper, Elisabeth C.

AU - Jones, Jonathan D.G.

AU - Maliga, Pal

PY - 1990/2

Y1 - 1990/2

N2 - The bacterial gene aad A encodes the enzyme aminoglycoside-3″-adenyltransferase that confers resistance to spectinomycin and streptomycin in Escherichia coli. Chimeric genes have been constructed for expression in plants, and were introduced into Nicotiana tabacum by Agrobacterium binary transformation vectors. Spectinomycin or streptomycin in selective concentrations prevent greening of N. tabacum calli. Transgenic clones, however, formed green calli on selective media containing spectinomycin, streptomycin, or both drugs. Resistance was inherited as a dominant Mendelian trait in the seed progeny. Resistance conferred by the chimeric aad A gene can be used as a color marker similar to the resistance conferred by the streptomycin phosphotransferase gene to streptomycin.

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KW - Aminoglycoside-3″-adenyltransferase

KW - Nicotiana tabacum

KW - chimeric gene

KW - spectinomycin resistance

KW - streptomycin resistance

KW - transgenic plant

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Aminoglycoside-3″-adenyltransferase confers resistance to spectinomycin and streptomycin in Nicotiana tabacum

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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