Aminoglycoside-3″-adenyltransferase confers resistance to spectinomycin and streptomycin in Nicotiana tabacum

Zora Svab, Elisabeth C. Harper, Jonathan D.G. Jones, Pal Maliga

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

The bacterial gene aad A encodes the enzyme aminoglycoside-3″-adenyltransferase that confers resistance to spectinomycin and streptomycin in Escherichia coli. Chimeric genes have been constructed for expression in plants, and were introduced into Nicotiana tabacum by Agrobacterium binary transformation vectors. Spectinomycin or streptomycin in selective concentrations prevent greening of N. tabacum calli. Transgenic clones, however, formed green calli on selective media containing spectinomycin, streptomycin, or both drugs. Resistance was inherited as a dominant Mendelian trait in the seed progeny. Resistance conferred by the chimeric aad A gene can be used as a color marker similar to the resistance conferred by the streptomycin phosphotransferase gene to streptomycin.

Original languageEnglish (US)
Pages (from-to)197-205
Number of pages9
JournalPlant Molecular Biology
Volume14
Issue number2
DOIs
StatePublished - Feb 1990
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Keywords

  • Aminoglycoside-3″-adenyltransferase
  • Nicotiana tabacum
  • chimeric gene
  • spectinomycin resistance
  • streptomycin resistance
  • transgenic plant

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