Amplifiable messenger RNA

Ying Wu, David Yong Zhang, Fred Russell Kramer

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

RNA molecules were prepared that consisted of an mRNA encoding chloramphenicol acetyltransferase embedded within the sequence of midivariant RNA, which is a template for the RNA-directed RNA polymerase Qβ replicase. These recombinant RNAs were shown to be bifunctional: they are amplified exponentially by incubation with Qβ replicase, and the replicated RNA serves as template for the cell-free synthesis of enzymatically active chloramphenicol acetyltransferase. The availability of amplifiable mRNAs will enable relatively large amounts of protein to be synthesized in vitro.

Original languageEnglish (US)
Pages (from-to)11769-11773
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number24
DOIs
StatePublished - 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • Biologically active chloramphenicol acetyltransferase
  • Cell-free protein synthesis
  • Exponential RNA synthesis
  • Qβ replicase
  • Recombinant RNA

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