An efficient protocol for VZV BAC-based mutagenesis

Zhen Zhang, Ying Huang, Hua Zhu

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Varicella-zoster virus (VZV) causes both varicella (chicken pox) and herpes zoster (shingles). As a member of the human herpesvirus family, VZV contains a large 125-kb DNA genome, encoding 70 unique open reading frames (ORFs). The genetic study of VZV has been hindered by the large size of viral genome, and thus the functions of the majority of these ORFs remain unclear. Recently, an efficient protocol has been developed based on a luciferase-containing VZV bacteria artificial chromosome (BAC) system to rapidly isolate and study VZV ORF deletion mutants.

Original languageEnglish (US)
Title of host publicationIn Vitro Mutagenesis Protocols
Subtitle of host publicationThird Edition
EditorsBraman Jeff
Pages75-86
Number of pages12
DOIs
StatePublished - Dec 1 2010

Publication series

NameMethods in Molecular Biology
Volume634
ISSN (Print)1064-3745

Fingerprint

Artificial Chromosomes
Human Herpesvirus 3
Mutagenesis
Bacteria
Open Reading Frames
Chickenpox
Herpes Zoster
Viral Genome
Herpesviridae
Luciferases
Genome
DNA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Bacterial artificial chromosome
  • Bioluminescence
  • Deletion mutagenesis
  • Varicella-zoster virus

Cite this

Zhang, Z., Huang, Y., & Zhu, H. (2010). An efficient protocol for VZV BAC-based mutagenesis. In B. Jeff (Ed.), In Vitro Mutagenesis Protocols: Third Edition (pp. 75-86). (Methods in Molecular Biology; Vol. 634). https://doi.org/10.1007/978-1-60761-652-8_5
Zhang, Zhen ; Huang, Ying ; Zhu, Hua. / An efficient protocol for VZV BAC-based mutagenesis. In Vitro Mutagenesis Protocols: Third Edition. editor / Braman Jeff. 2010. pp. 75-86 (Methods in Molecular Biology).
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Zhang, Z, Huang, Y & Zhu, H 2010, An efficient protocol for VZV BAC-based mutagenesis. in B Jeff (ed.), In Vitro Mutagenesis Protocols: Third Edition. Methods in Molecular Biology, vol. 634, pp. 75-86. https://doi.org/10.1007/978-1-60761-652-8_5

An efficient protocol for VZV BAC-based mutagenesis. / Zhang, Zhen; Huang, Ying; Zhu, Hua.

In Vitro Mutagenesis Protocols: Third Edition. ed. / Braman Jeff. 2010. p. 75-86 (Methods in Molecular Biology; Vol. 634).

Research output: Chapter in Book/Report/Conference proceedingChapter

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AB - Varicella-zoster virus (VZV) causes both varicella (chicken pox) and herpes zoster (shingles). As a member of the human herpesvirus family, VZV contains a large 125-kb DNA genome, encoding 70 unique open reading frames (ORFs). The genetic study of VZV has been hindered by the large size of viral genome, and thus the functions of the majority of these ORFs remain unclear. Recently, an efficient protocol has been developed based on a luciferase-containing VZV bacteria artificial chromosome (BAC) system to rapidly isolate and study VZV ORF deletion mutants.

KW - Bacterial artificial chromosome

KW - Bioluminescence

KW - Deletion mutagenesis

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Zhang Z, Huang Y, Zhu H. An efficient protocol for VZV BAC-based mutagenesis. In Jeff B, editor, In Vitro Mutagenesis Protocols: Third Edition. 2010. p. 75-86. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-60761-652-8_5