An essential role for Arabidopsis Trs33 in cell growth and organization in plant apical meristems

Jin Zhang, Jun Chen, Lijuan Wang, Shutang Zhao, Weina Wang, Jianbo Li, Bobin Liu, Xingyun Qi, Huanquan Zheng, Mengzhu Lu

Research output: Contribution to journalArticle

Abstract

Key message: Trafficking protein particle (TRAPP) complexes subunit gene AtTrs33 plays an important role in keeping apical meristematic activity and dominance in Arabidopsis. Abstract: TRAPP complexes, composed of multimeric subunits, are guanine–nucleotide exchange factors for certain Rab GTPases and are believed to be involved in the regulation of membrane trafficking, but the cases in Arabidopsis are largely unknown. Trs33, recently proposed to be a component of TRAPP IV, is non-essential in yeast cells. A single copy of Trs33 gene, AtTrs33, was identified in Arabidopsis. GUS activity assay indicated that AtTrs33 was ubiquitously expressed. Based on a T-DNA insertion line, we found that loss-of-function of AtTrs33 is lethal for apical growth. Knock-down or knock-in of AtTrs33 affects apical meristematic growth and fertility, which indicates that AtTrs33 plays an important role in keeping apical meristematic activity and dominance in Arabidopsis. Analysis of auxin responses and PIN1/2 localization indicate that impaired apical meristematic activity and dominance were caused by altered auxin responses through non-polarized PIN1 localization. The present study reported that AtTrs33 plays an essential role in Arabidopsis cell growth and organization, which is different with its homologue in yeast. These findings provide new insights into the functional divergence of TRAPP subunits.

Original languageEnglish (US)
Pages (from-to)381-391
Number of pages11
JournalPlant Cell Reports
Volume39
Issue number3
DOIs
StatePublished - Mar 1 2020

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All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Keywords

  • Apical meristem
  • Arabidopsis
  • Auxin
  • PIN1/2
  • Subcellular localization
  • TRAPP
  • Trs33

Cite this

Zhang, J., Chen, J., Wang, L., Zhao, S., Wang, W., Li, J., Liu, B., Qi, X., Zheng, H., & Lu, M. (2020). An essential role for Arabidopsis Trs33 in cell growth and organization in plant apical meristems. Plant Cell Reports, 39(3), 381-391. https://doi.org/10.1007/s00299-019-02497-9