@article{6cce660d85a446c789944d7eeab3f25b,
title = "An Essential Role for RIG-I in Toll-like Receptor-Stimulated Phagocytosis",
abstract = "Retinoic acid-inducible gene-I (RIG-I) plays an important role in antiviral response by recognizing double-stranded RNA. Here we demonstrate an unanticipated role of RIG-I in Toll-like receptor (TLR)-stimulated phagocytosis. Stimulation with lipopolysaccharide (LPS), a ligand of TLR4, induced the expression of RIG-I in macrophages. Depletion of RIG-I by RNAi or gene targeting inhibited the LPS-induced phagocytosis of bacteria. Cellular processes involved in phagocytosis, such as small GTPase Cdc42/Rac1 activation, actin polymerization, and actin-regulator Arp2/3 recruitment, were also impaired in RIG-I-deficient macrophages activated by LPS. Moreover, RIG-I-/- mice were found to be more susceptible to infection with Escherichia coli as compared to wild-type mice. Thus, the regulatory functions of RIG-I are strikingly broad, including a role not only in antiviral responses but in antibacterial responses as well.",
keywords = "CELLBIO, MICROBIO, MOLIMMUNO",
author = "Ling Kong and Lei Sun and Hongxin Zhang and Qin Liu and Ye Liu and Linhua Qin and Guojun Shi and Hu, {Jun Hao} and Ajing Xu and Sun, {Yue Ping} and Dangsheng Li and Shi, {Yu Fang} and Zang, {Jing Wu} and Jiang Zhu and Zhu Chen and Wang, {Zhu Gang} and Ge, {Bao Xue}",
note = "Funding Information: We thank Drs. J.L. Strominger, R. Medzhitov, G. Cheng, Y.W. He, C. Dong, and S.M. Skinner for critical reading of the manuscript; Dr. Christina Mitchell for kindly providing Cdc42/Rac plasmids; and Dr. Takashi Fujita for kindly providing RIG-I plasmids. This work is supported by the Ministry of Science and Technology Grants 2006CB504300 and 2009ZX10004-308, the National Natural Science Foundation of China (projects 30525012, 30671914, and 30471580), Shanghai E-Research Institutes, and Science and Technology Commission of Shanghai Municipality (project 04DZ14902 and 07pj14096). L.K. and L.S. designed and did most experiments, analyzed data, and prepared the manuscript; Q.L., Y.L., L.Q., G.S., H.Z., and J.-H.H. did the experiments; A.X. and Y.-P.S. assisted with experiments; D.L., Y.-F.S., and J.-W.Z. prepared the manuscript; J.Z., Z.C., and Z.-G.W. provided materials; and B.-X.G. conceptualized the research, directed the study, and prepared the manuscript. ",
year = "2009",
month = aug,
day = "20",
doi = "10.1016/j.chom.2009.06.008",
language = "English (US)",
volume = "6",
pages = "150--161",
journal = "Cell Host and Microbe",
issn = "1931-3128",
publisher = "Cell Press",
number = "2",
}