An in vitro method to study the effects of hematopoietic regulators during immune and blood cell development

Nitixa Patel, Marianne Castillo, Pranela Rameshwar

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

In adults, hematopoiesis occurs in bone marrow (BM) through a complex process with differentiation of hematopoietic stem cells (HSCs) to immune and blood cells. Human HSCs and their progenitors express CD34. Methods on hematopoietic regulation are presented to show the effects of the chemokine, stromal-derived growth factor (SDF)-1α and the neuropeptide, substance P (SP). SDF-1α production in BM stroma causes interactions with HSCs, thereby retaining the HSCs in regions close to the endosteum, at low oxygen. Small changes in SDF-1α levels stimulate HSC functions through direct and indirect mechanisms. The indirect method occurs by SP production, which stimulates CD34+ cells, supported by ligand-binding studies, long-term culture-initiating cell assays for HSC functions, and clonogenic assays for myeloid progenitors. These methods can be applied to study other hematopoietic regulators.

Original languageEnglish (US)
Pages (from-to)56-64
Number of pages9
JournalBiological Procedures Online
Volume9
Issue number1
DOIs
StatePublished - 2007

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Keywords

  • Cell culture techniques
  • Cytokines
  • Hematopoiesis
  • Hematopoietic stem cells

Fingerprint

Dive into the research topics of 'An in vitro method to study the effects of hematopoietic regulators during immune and blood cell development'. Together they form a unique fingerprint.

Cite this