An in vitro system for the investigation of heterologous RNA recombination

Xueying Qiao, Jian Qiao, Leonard Mindich

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Bacteriophage Φ6 has a genome of three segments of double-stranded RNA enclosed in a polyhedral procapsid. Purified procapsids are capable of the specific packaging of viral plus strands and the synthesis of their complementary minus strands. The genomic segments of Φ6 are capable of heterologous recombination. We have prepared an in vitro system containing purified procapsids that is capable of packaging plus strands of the genomic segments and synthesizing minus strands on these templates. The system generates heterologous recombination products when stimulated by having one of the plus strands incapable of serving as a template for minus strand synthesis. Recombinants were produced upon transfection of spheroplasts with the in vitro packaged and replicated RNA. Sites of recombination were not found to be localized in particular regions of either the donor or the recipient strands.

Original languageEnglish (US)
Pages (from-to)103-110
Number of pages8
JournalVirology
Volume227
Issue number1
DOIs
StatePublished - Jan 6 1997

All Science Journal Classification (ASJC) codes

  • Virology

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