An invertase with unusual properties secreted by sucrose-grown cells of Corynebacterium murisepticum.

M. A. Nadkarni, V. N. Pandey, D. S. Pradhan

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The mode of sucrose utilisation by Corynebacterium murisepticum cells growing on M9 minimal medium supplemented with 0.4% sucrose as the carbon source was studied. It was observed that during growth of this organism, sucrose in the medium is hydrolysed to glucose and fructose, suggesting the formation of an extracellular invertase. Unlike in other microorganisms (e.g. Saccharomyces cerevisiae) the invertase formation is not repressed by the presence of glucose in the medium. The invertase was found to be the only predominant extracellular protein in the culture broth and could be purified in a single step by precipitation at 90% ammonium sulphate saturation. The purified protein had a molecular mass of 70,000 daltons. It not only showed invertase activity, but also a fructosyltransferase activity as it could convert sucrose to beta-1,2-difructose, as well as to glucose and fructose.

Original languageEnglish (US)
Pages (from-to)156-159
Number of pages4
JournalIndian journal of biochemistry & biophysics
Volume30
Issue number3
StatePublished - Jun 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry

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