Analysis of an improved Cyanophora paradoxa genome assembly

Dana C. Price, Ursula W. Goodenough, Robyn Roth, Jae Hyeok Lee, Thamali Kariyawasam, Marek Mutwil, Camilla Ferrari, Fabio Facchinelli, Steven G. Ball, Ugo Cenci, Cheong Xin Chan, Nicole E. Wagner, Hwan Su Yoon, Andreas P.M. Weber, Debashish Bhattacharya

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Glaucophyta are members of the Archaeplastida, the founding group of photosynthetic eukaryotes that also includes red algae (Rhodophyta), green algae, and plants (Viridiplantae). Here we present a high-quality assembly, built using long-read sequences, of the ca. 100 Mb nuclear genome of the model glaucophyte Cyanophora paradoxa. We also conducted a quick-freeze deep-etch electron microscopy (QFDEEM) analysis of C. paradoxa cells to investigate glaucophyte morphology in comparison to other organisms. Using the genome data, we generated a resolved 115-taxon eukaryotic tree of life that includes a well-supported, monophyletic Archaeplastida. Analysis of muroplast peptidoglycan (PG) ultrastructure using QFDEEM shows that PG is most dense at the cleavage-furrow. Analysis of the chlamydial contribution to glaucophytes and other Archaeplastida shows that these foreign sequences likely played a key role in anaerobic glycolysis in primordial algae to alleviate ATP starvation under night-time hypoxia. The robust genome assembly of C. paradoxa significantly advances knowledge about this model species and provides a reference for exploring the panoply of traits associated with the anciently diverged glaucophyte lineage.

Original languageEnglish (US)
Pages (from-to)287-299
Number of pages13
JournalDNA Research
Issue number4
StatePublished - Aug 1 2019

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


  • Archaeplastida
  • Cyanophora paradoxa
  • phylogenomics
  • tree of eukaryotes
  • ultrastructure


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