Analysis of hOGG1 genotype as a prognostic marker for muscle invasive bladder cancer: A novel approach using peptide nucleic acid-mediated, real-time PCR clamping

Eun Jung Kim, Chunri Yan, Yun Sok Ha, Pildu Jeong, Isaac Kim, Sung Kwon Moon, Yung Hyun Choi, Wun Jae Kim

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Objective: DNA damage repair mechanisms are a source of genetic mutation and are believed to play an important role in human cancer. Human 8-oxoguanine DNA glycosylase 1 (hOGG1) is involved in the recognition and repair of DNA damage. The value of the hOGG1 genotype as a prognostic indicator for bladder cancer (BC) was assessed using a novel technological approach. Materials and methods: The association between genetic polymorphisms of hOGG1 codon 326 and clinicopathologic characteristics of 337 patients with BC was analyzed using peptide nucleic acid (PNA)-mediated real-time PCR clamping. Results: Tumor grade and size were significantly associated with the hOGG1 codon 326 genotype in non-muscle-invasive bladder cancer (NMIBC). The Cys326Cys polymorphism was significantly associated with progression and cancer specific survival in patients with muscle-invasive bladder cancer (MIBC). Multivariate Cox regression analysis indicated that the hOGG1 Cys326Cys polymorphism is associated with a protective effect on progression and a more dominant survival benefit than the Ser326Ser polymorphism in MIBC (hazard ratio 0.284 and 0.305, respectively). Conclusions: Analysis of genotypes and clinical data for 337 BC patients indicates that the hOGG1 genotype may be a useful prognostic genetic marker for MIBC.

Original languageEnglish (US)
Pages (from-to)673-679
Number of pages7
JournalUrologic Oncology: Seminars and Original Investigations
Volume30
Issue number5
DOIs
StatePublished - Sep 1 2012

All Science Journal Classification (ASJC) codes

  • Oncology
  • Urology

Keywords

  • Biological tumor markers
  • DNA damage repair mechanisms
  • HOGG1
  • PNA
  • Prognosis
  • Urinary bladder neoplasms

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