Analysis of microRNA effector functions in vitro

Bingbing Wang, John G. Doench, Carl D. Novina

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Analyses of gene functions in vitro provide the means to dissect biological phenomena. Development of cell-free assays for transcription, splicing, and translation has yielded great insights into macromolecular interactions and functions required for these processes. This article discusses development of cell-free assays to test macromolecular interactions and activities required for microRNA effector functions. This chapter also compares in vitro analyses to complementary studies in cells and the technical considerations that permit molecular analysis of microRNA function in cell-free conditions.

Original languageEnglish (US)
Pages (from-to)91-104
Number of pages14
Issue number2
StatePublished - Oct 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)


  • Cell-free
  • Gene silencing
  • MicroRNA
  • RNA interference
  • Translation
  • siRNA


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