Analysis of microRNA effector functions in vitro

Bingbing Wang; John G. Doench; Carl D. Novina

doi:10.1016/j.ymeth.2007.04.003

Analysis of microRNA effector functions in vitro

Bingbing Wang, John G. Doench, Carl D. Novina

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Analyses of gene functions in vitro provide the means to dissect biological phenomena. Development of cell-free assays for transcription, splicing, and translation has yielded great insights into macromolecular interactions and functions required for these processes. This article discusses development of cell-free assays to test macromolecular interactions and activities required for microRNA effector functions. This chapter also compares in vitro analyses to complementary studies in cells and the technical considerations that permit molecular analysis of microRNA function in cell-free conditions.

Original language	English (US)
Pages (from-to)	91-104
Number of pages	14
Journal	Methods
Volume	43
Issue number	2
DOIs	https://doi.org/10.1016/j.ymeth.2007.04.003
State	Published - Oct 2007
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)

Keywords

Cell-free
Gene silencing
MicroRNA
RNA interference
Translation
siRNA

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10.1016/j.ymeth.2007.04.003

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abstract = "Analyses of gene functions in vitro provide the means to dissect biological phenomena. Development of cell-free assays for transcription, splicing, and translation has yielded great insights into macromolecular interactions and functions required for these processes. This article discusses development of cell-free assays to test macromolecular interactions and activities required for microRNA effector functions. This chapter also compares in vitro analyses to complementary studies in cells and the technical considerations that permit molecular analysis of microRNA function in cell-free conditions.",

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AB - Analyses of gene functions in vitro provide the means to dissect biological phenomena. Development of cell-free assays for transcription, splicing, and translation has yielded great insights into macromolecular interactions and functions required for these processes. This article discusses development of cell-free assays to test macromolecular interactions and activities required for microRNA effector functions. This chapter also compares in vitro analyses to complementary studies in cells and the technical considerations that permit molecular analysis of microRNA function in cell-free conditions.

KW - Cell-free

KW - Gene silencing

KW - MicroRNA

KW - RNA interference

KW - Translation

KW - siRNA

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ER -

Analysis of microRNA effector functions in vitro

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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