Analysis of two transcription activation elements in the promoter of the developmentally regulated con-10 gene of Neurospora crassa

Kwangwon Lee, Daniel J. Ebbole

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The con-10 gene of Neurospora crassa is activated during conidiation. CRS-B (conidiation response sequence-B) and CGE (con-10 general enhancer) elements have been proposed to function as sites of transcriptional activation and as possible elements that confer developmental regulation to con-10. In a specific analysis of the roles of these elements we found that two CRS-B elements are necessary for full activation of con-10 during macroconidiation, whereas two CGE elements are functionally redundant, such that a single CGE is sufficient for maximal expression. However, CRS-B and CGE elements are not sufficient for developmental activation of a reporter gene. The CRS-B element was further dissected and one of the CRS-B elements appears to function in repression as well as activation, con-10 is also highly expressed during microconidiation, a different form of asexual sporulation, but we show here that CRS-B elements do not play a significant role in con-10 expression during microconidiation. Both CRS-B elements contribute to basal con-10 expression during mycelial growth, con-10 is also regulated by light, and CRS-B and CGE elements may play minor roles in controlling con-10 expression in response to light.

Original languageEnglish (US)
Pages (from-to)259-268
Number of pages10
JournalFungal Genetics and Biology
Volume23
Issue number3
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

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Neurospora crassa
Transcriptional Activation
Genes
Light
Reporter Genes
Growth

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Genetics

Cite this

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abstract = "The con-10 gene of Neurospora crassa is activated during conidiation. CRS-B (conidiation response sequence-B) and CGE (con-10 general enhancer) elements have been proposed to function as sites of transcriptional activation and as possible elements that confer developmental regulation to con-10. In a specific analysis of the roles of these elements we found that two CRS-B elements are necessary for full activation of con-10 during macroconidiation, whereas two CGE elements are functionally redundant, such that a single CGE is sufficient for maximal expression. However, CRS-B and CGE elements are not sufficient for developmental activation of a reporter gene. The CRS-B element was further dissected and one of the CRS-B elements appears to function in repression as well as activation, con-10 is also highly expressed during microconidiation, a different form of asexual sporulation, but we show here that CRS-B elements do not play a significant role in con-10 expression during microconidiation. Both CRS-B elements contribute to basal con-10 expression during mycelial growth, con-10 is also regulated by light, and CRS-B and CGE elements may play minor roles in controlling con-10 expression in response to light.",
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Analysis of two transcription activation elements in the promoter of the developmentally regulated con-10 gene of Neurospora crassa. / Lee, Kwangwon; Ebbole, Daniel J.

In: Fungal Genetics and Biology, Vol. 23, No. 3, 01.01.1998, p. 259-268.

Research output: Contribution to journalArticle

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