TY - JOUR
T1 - Analytical aspects of population-specific DNA fingerprinting for individuals
AU - Smouse, P. E.
AU - Chevillon, C.
N1 - Funding Information:
From the Center for Theoretical and Applied Genetics, Cook College, Rutgers University, New Brunswick, NJ 08903–0231 (Smouse) and the Institut des Sciences de l’Evolution, Universite de Montpellier II, Montpellier, France (Chevillon). The authors wish to thank S. Kaufman, C. J. Kobak, B. S. Weir, and an anonymous reviewer, all of whom contributed helpful critique on the manuscript. The work was supported by the New Jersey Agricultural Experiment Station, USDA-NJAES-32106. Address correspondence to P. Smouse at the address above or e-mail: smouse@aesop.rutgers.edu.
PY - 1998/3
Y1 - 1998/3
N2 - An emerging problem of some interest is whether we can determine the population membership of a single individual, using a population-specific 'genetic fingerprint.' The levels of accuracy and precision required are beyond the reach of allozyme analysis, and attention has shifted to DNA polymorphisms. There are different types of DNA markers available for population surveys: RFLPs, mini- and microsatellites, and RAPDs, and each type has its own strengths and weaknesses. We present a generic analysis that relates gene pool separation to our ability to assign individuals, an analysis that does not depend on the type of marker. We then review strengths and weaknesses of different DNA markers, in the context of DNA fingerprinting. Codominant loci are best. It is possible to gain more information per marker with multlallelic loci, but diminishing returns set in rapidly, and it is better to add loci. A modest number of independent loci is best, each locus with a modest number of alleles and with each allele in modest frequency.
AB - An emerging problem of some interest is whether we can determine the population membership of a single individual, using a population-specific 'genetic fingerprint.' The levels of accuracy and precision required are beyond the reach of allozyme analysis, and attention has shifted to DNA polymorphisms. There are different types of DNA markers available for population surveys: RFLPs, mini- and microsatellites, and RAPDs, and each type has its own strengths and weaknesses. We present a generic analysis that relates gene pool separation to our ability to assign individuals, an analysis that does not depend on the type of marker. We then review strengths and weaknesses of different DNA markers, in the context of DNA fingerprinting. Codominant loci are best. It is possible to gain more information per marker with multlallelic loci, but diminishing returns set in rapidly, and it is better to add loci. A modest number of independent loci is best, each locus with a modest number of alleles and with each allele in modest frequency.
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U2 - 10.1093/jhered/89.2.143
DO - 10.1093/jhered/89.2.143
M3 - Article
C2 - 9542162
AN - SCOPUS:0031978467
SN - 0022-1503
VL - 89
SP - 143
EP - 150
JO - Journal of Heredity
JF - Journal of Heredity
IS - 2
ER -