Analytical methods to determine phytoestrogenic compounds

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75 Scopus citations

Abstract

The analytical methods for the determination of phytoestrogenic compounds in edible plants, plant products and biological matrices are reviewed. The detection, qualitative and quantitative methods based on different chromatographic separations of gas chromatography (GC), high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) coupled with various detections by ultraviolet absorption (UV), electrochemical detection (ED), fluorescence detection, mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR), as well as non-chromatographic immunoassay are each extensively examined and compared. An overview on phytoestrogen chemistry, bioactivities and health effects, plant precursors, metabolism and sample preparation is also presented.

Original languageEnglish (US)
Pages (from-to)325-355
Number of pages31
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume812
Issue number1-2 SPEC. ISS.
DOIs
StatePublished - Dec 5 2004

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Keywords

  • Analytical method
  • Isoflavone
  • Lignan
  • Phytoestrogen
  • Review

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