Analyzing mouse neural stem cell and progenitor cell proliferation using EdU incorporation and multicolor flow cytometry

Fernando Janczur Velloso, Ekta Kumari, Krista D. Buono, Michelle J. Frondelli, Steven W. Levison

Research output: Contribution to journalArticlepeer-review

Abstract

This protocol describes an ex vivo approach to identify and quantify the proportions of proliferating neural stem cells and progenitors of the mouse subventricular zone. It uses ethynyl deoxyuridine (EdU) incorporation to identify dividing cells, combined with multicolor flow cytometry for 4 cell surface antigens to distinguish between 8 phenotypically distinct mouse neural progenitors and stem cells. It has been optimized for wild-type neonatal mice but can be used on mice of any postnatal age. For complete details on the use and execution of this profile, please refer to Kumari et al. (2020).

Original languageEnglish (US)
Article number101065
JournalSTAR Protocols
Volume3
Issue number1
DOIs
StatePublished - Mar 18 2022

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)

Keywords

  • Cell Biology
  • Cell isolation
  • Developmental biology
  • Flow Cytometry/Mass Cytometry
  • Neuroscience
  • Stem Cells

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