Angiotensin II effect on 22Na+ transport in vascular smooth muscle cells

S. Kuriyama, A. Nakamura, L. Hopp, B. P. Fine, M. Kino, E. Cragoe, A. Aviv

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13 Scopus citations


It is well established that angiotensin II (AII) rapidly increases free cytosolic Ca2+ in vascular smooth muscle cells (VSMCs). Several studies have indicated that the hormone also plays a role in Na+-K+ regulation of these cells. In this study, we explored the mechanism of AII effect on 22Na+ transport in cultured rat VSMCs. The 22Na+ washout from these cells was described by three exponents with exponential factors k1>k2>k3. In 1.8 mM Ca2+ medium, AII (10-9-10-6M) increased (in a dose response manner) the k1 value, and consequently the initial washout rate constant (ki(e)) for the isotope. AII had no effect on ki(e) in Ca2+-deficient medium or in the presence of ouabain. Amiloride (10-3 M) and verapamil (10-5M) abolished the AII induced increase in ki(e). These findings are consistent with angiotensin II stimulation of an amiloride-sensitive Na+ transport, which is likely to represent the Na+/H+ antiport. In cultured VSMCs, the sustained stimulation by AII of this transport system requires the presence of extracellular Ca2+ and its influx into these cells.

Original languageEnglish (US)
Pages (from-to)139-146
Number of pages8
JournalJournal of cardiovascular pharmacology
Issue number2
StatePublished - 1988

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Cardiology and Cardiovascular Medicine


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