Antcin K, an active triterpenoid from the fruiting bodies of basswood-cultivated antrodia cinnamomea, inhibits metastasis via suppression of integrin-mediated adhesion, migration, and invasion in human hepatoma cells

Ya Ling Huang, Yung Lin Chu, Chi-Tang Ho, Jing Gung Chung, Chiao I. Lai, Yu Cheng Su, Yueh Hsiung Kuo, Lee Yan Sheen

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Previous research demonstrated that the ethyl acetate extract from Antrodia cinnamomea suppresses the invasive potential of human breast and hepatoma cells, but the effective compounds are not identified. The main bioactive compounds of A. cinnamomea are ergostane-type triterpenoids, and the content of antcin K is the highest. The objective of this study was to evaluate the antimetastatic activity and mechanisms of antcin K purified from the fruiting body of basswood-cultivated A. cinnamomea on human liver cancer Hep 3B cells. The results showed that adhesion, migration, and invasion of Hep 3B cells were effectively inhibited by antcin K within 24 h of treatment. Antcin K not only reduced the protein expression and activity of MMP-2 and MMP-9 but also down-regulated vimentin and up-regulated E-cadherin in Hep 3B cells. In depth investigation for the molecular mechanism revealed that antcin K could reduce the protein expression of integrin β1, β3, α5, and αv and suppress phosphorylation of FAK, Src, PI3K, AKT, MEK, ERK, and JNK. These results suggested that antcin K was able to inhibit the metastasis of human hepatoma cells through suppression of integrin-mediated adhesion, migration, and invasion. Coupled with these findings, antcin K has a good potential to reduce the risk of liver cancer metastasis.

Original languageEnglish (US)
Pages (from-to)4561-4569
Number of pages9
JournalJournal of agricultural and food chemistry
Volume63
Issue number18
DOIs
StatePublished - May 13 2015

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Keywords

  • Antrodia cinnamomea
  • antcin K
  • epithelial mesenchymal transition (EMT)
  • liver cancer metastasis
  • matrix metalloproteinases (MMPs)

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