Antibiofilm Activity of Actinobacillus pleuropneumoniae Serotype 5 Capsular Polysaccharide

Michael T. Karwacki, Daniel E. Kadouri, Meriem Bendaoud, Era A. Izano, Vandana Sampathkumar, Thomas J. Inzana, Jeffrey B. Kaplan

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Cell-free extracts isolated from colony biofilms of Actinobacillus pleuropneumoniae serotype 5 were found to inhibit biofilm formation by Staphylococcus aureus, S. epidermidis and Aggregatibacter actinomycetemcomitans, but not by A. pleuropneumoniae serotype 5 itself, in a 96-well microtiter plate assay. Physical and chemical analyses indicated that the antibiofilm activity in the extract was due to high-molecular-weight polysaccharide. Extracts isolated from a mutant strain deficient in the production of serotype 5 capsular polysaccharide did not exhibit antibiofilm activity. A plasmid harboring the serotype 5 capsule genes restored the antibiofilm activity in the mutant extract. Purified serotype 5 capsular polysaccharide also exhibited antibiofilm activity against S. aureus. A. pleuropneumoniae wild-type extracts did not inhibit S. aureus growth, but did inhibit S. aureus intercellular adhesion and binding of S. aureus cells to stainless steel surfaces. Furthermore, polystyrene surfaces coated with A. pleuropneumoniae wild-type extracts, but not with capsule-mutant extracts, resisted S. aureus biofilm formation. Our findings suggest that the A. pleuropneumoniae serotype 5 capsule inhibits cell-to-cell and cell-to-surface interactions of other bacteria. A. pleuropneumoniae serotype 5 capsular polysaccharide is one of a growing number of bacterial polysaccharides that exhibit broad-spectrum, nonbiocidal antibiofilm activity. Future studies on these antibiofilm polysaccharides may uncover novel functions for bacterial polysaccharides in nature, and may lead to the development of new classes of antibiofilm agents for industrial and clinical applications.

Original languageEnglish (US)
Article numbere63844
JournalPloS one
Volume8
Issue number5
DOIs
StatePublished - May 14 2013

Fingerprint

Actinobacillus pleuropneumoniae 5
Actinobacillus pleuropneumoniae
Polysaccharides
polysaccharides
Staphylococcus aureus
Biofilms
Bacterial Polysaccharides
extracts
Capsules
biofilm
serotypes
mutants
cells
Actinobacillus actinomycetemcomitans
Polystyrenes
Stainless Steel
surface interactions
Aggregatibacter actinomycetemcomitans
Staphylococcus epidermidis
Assays

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Karwacki, M. T., Kadouri, D. E., Bendaoud, M., Izano, E. A., Sampathkumar, V., Inzana, T. J., & Kaplan, J. B. (2013). Antibiofilm Activity of Actinobacillus pleuropneumoniae Serotype 5 Capsular Polysaccharide. PloS one, 8(5), [e63844]. https://doi.org/10.1371/journal.pone.0063844
Karwacki, Michael T. ; Kadouri, Daniel E. ; Bendaoud, Meriem ; Izano, Era A. ; Sampathkumar, Vandana ; Inzana, Thomas J. ; Kaplan, Jeffrey B. / Antibiofilm Activity of Actinobacillus pleuropneumoniae Serotype 5 Capsular Polysaccharide. In: PloS one. 2013 ; Vol. 8, No. 5.
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Karwacki, MT, Kadouri, DE, Bendaoud, M, Izano, EA, Sampathkumar, V, Inzana, TJ & Kaplan, JB 2013, 'Antibiofilm Activity of Actinobacillus pleuropneumoniae Serotype 5 Capsular Polysaccharide', PloS one, vol. 8, no. 5, e63844. https://doi.org/10.1371/journal.pone.0063844

Antibiofilm Activity of Actinobacillus pleuropneumoniae Serotype 5 Capsular Polysaccharide. / Karwacki, Michael T.; Kadouri, Daniel E.; Bendaoud, Meriem; Izano, Era A.; Sampathkumar, Vandana; Inzana, Thomas J.; Kaplan, Jeffrey B.

In: PloS one, Vol. 8, No. 5, e63844, 14.05.2013.

Research output: Contribution to journalArticle

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AU - Karwacki, Michael T.

AU - Kadouri, Daniel E.

AU - Bendaoud, Meriem

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AU - Sampathkumar, Vandana

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AU - Kaplan, Jeffrey B.

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