Antigenic signature analysis reflects differences among plant virus isolates

Rong Di, John H. Hill, Richard A. Van Deusen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Antigenic differences among cowpea severe mosaic virus (CPSMV) isolates were clearly reflected in signature analysis employing a panel of seven well-characterized, monoclonal antibodies. Separate binding curves were generated by reacting serial dilutions of extracts from infected plant tissue containing each antigen simultaneously with each antibody in the panel. An iterative procedure was used to align unknown CPSMV antigen concentrations from different antigen preparations to allow comparison of binding profiles from different assays. Signature analysis was shown to be highly useful for the elucidation of subtle antigenic differences among viral agents because it requires neither purified virus nor knowledge of virus concentration in sap from infected plants.

Original languageEnglish (US)
Pages (from-to)281-291
Number of pages11
JournalJournal of Virological Methods
Volume42
Issue number2-3
DOIs
StatePublished - Jan 1 1993

Fingerprint

Plant Viruses
Comovirus
Antigens
Viruses
Plant Extracts
Monoclonal Antibodies
Antibodies

All Science Journal Classification (ASJC) codes

  • Virology

Keywords

  • Comovirus
  • Cowpea severe mosaic virus
  • Diagnosis

Cite this

Di, Rong ; Hill, John H. ; Van Deusen, Richard A. / Antigenic signature analysis reflects differences among plant virus isolates. In: Journal of Virological Methods. 1993 ; Vol. 42, No. 2-3. pp. 281-291.
@article{d04109db1318441b84d11171d8608f99,
title = "Antigenic signature analysis reflects differences among plant virus isolates",
abstract = "Antigenic differences among cowpea severe mosaic virus (CPSMV) isolates were clearly reflected in signature analysis employing a panel of seven well-characterized, monoclonal antibodies. Separate binding curves were generated by reacting serial dilutions of extracts from infected plant tissue containing each antigen simultaneously with each antibody in the panel. An iterative procedure was used to align unknown CPSMV antigen concentrations from different antigen preparations to allow comparison of binding profiles from different assays. Signature analysis was shown to be highly useful for the elucidation of subtle antigenic differences among viral agents because it requires neither purified virus nor knowledge of virus concentration in sap from infected plants.",
keywords = "Comovirus, Cowpea severe mosaic virus, Diagnosis",
author = "Rong Di and Hill, {John H.} and {Van Deusen}, {Richard A.}",
year = "1993",
month = "1",
day = "1",
doi = "10.1016/0166-0934(93)90039-T",
language = "English (US)",
volume = "42",
pages = "281--291",
journal = "Journal of Virological Methods",
issn = "0166-0934",
publisher = "Elsevier",
number = "2-3",

}

Antigenic signature analysis reflects differences among plant virus isolates. / Di, Rong; Hill, John H.; Van Deusen, Richard A.

In: Journal of Virological Methods, Vol. 42, No. 2-3, 01.01.1993, p. 281-291.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antigenic signature analysis reflects differences among plant virus isolates

AU - Di, Rong

AU - Hill, John H.

AU - Van Deusen, Richard A.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Antigenic differences among cowpea severe mosaic virus (CPSMV) isolates were clearly reflected in signature analysis employing a panel of seven well-characterized, monoclonal antibodies. Separate binding curves were generated by reacting serial dilutions of extracts from infected plant tissue containing each antigen simultaneously with each antibody in the panel. An iterative procedure was used to align unknown CPSMV antigen concentrations from different antigen preparations to allow comparison of binding profiles from different assays. Signature analysis was shown to be highly useful for the elucidation of subtle antigenic differences among viral agents because it requires neither purified virus nor knowledge of virus concentration in sap from infected plants.

AB - Antigenic differences among cowpea severe mosaic virus (CPSMV) isolates were clearly reflected in signature analysis employing a panel of seven well-characterized, monoclonal antibodies. Separate binding curves were generated by reacting serial dilutions of extracts from infected plant tissue containing each antigen simultaneously with each antibody in the panel. An iterative procedure was used to align unknown CPSMV antigen concentrations from different antigen preparations to allow comparison of binding profiles from different assays. Signature analysis was shown to be highly useful for the elucidation of subtle antigenic differences among viral agents because it requires neither purified virus nor knowledge of virus concentration in sap from infected plants.

KW - Comovirus

KW - Cowpea severe mosaic virus

KW - Diagnosis

UR - http://www.scopus.com/inward/record.url?scp=0027254136&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027254136&partnerID=8YFLogxK

U2 - 10.1016/0166-0934(93)90039-T

DO - 10.1016/0166-0934(93)90039-T

M3 - Article

C2 - 7685770

AN - SCOPUS:0027254136

VL - 42

SP - 281

EP - 291

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

IS - 2-3

ER -