Antigenic signature analysis reflects differences among plant virus isolates

Rong Di, John H. Hill, Richard A. Van Deusen

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Antigenic differences among cowpea severe mosaic virus (CPSMV) isolates were clearly reflected in signature analysis employing a panel of seven well-characterized, monoclonal antibodies. Separate binding curves were generated by reacting serial dilutions of extracts from infected plant tissue containing each antigen simultaneously with each antibody in the panel. An iterative procedure was used to align unknown CPSMV antigen concentrations from different antigen preparations to allow comparison of binding profiles from different assays. Signature analysis was shown to be highly useful for the elucidation of subtle antigenic differences among viral agents because it requires neither purified virus nor knowledge of virus concentration in sap from infected plants.

Original languageEnglish (US)
Pages (from-to)281-291
Number of pages11
JournalJournal of Virological Methods
Volume42
Issue number2-3
DOIs
Publication statusPublished - May 1993

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Virology

Keywords

  • Comovirus
  • Cowpea severe mosaic virus
  • Diagnosis

Cite this