Antigenicity of Campylobacter jejuni flagella

Martin J. Blaser, Janet A. Hopkins, Guillermo I. Perez-Perez, Henry J. Cody, Diane G. Newell

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We studied the antigenicity of a wild-type flagellate and motile (F+M+) Campylobacter jejuni strain (81116) and two daughter mutants, one flagellate and immotile (F+M-) and one aflagellate and immotile (F-M-). By sodium dodecyl sulfate-polyacrylamide gel electrophoresis of acid-extracted surface proteins, a 63-kilodalton (kDa) band identified from sheared flagella as the flagella protein was present in the F+M+ and F+M- strains but not in the F-M- strain. No other differences in protein profile among the three strains were noted. By Western blotting, serum from rabbits immunized with either the F+M+ or F-M- strain detected a 63-kDa protein in the F+M+ and F+M- strains but not in the F-M- strain. That the F-M- antiserum recognized the 63-kDa band suggests that small amounts of this protein or a cross-reacting antigen is present on the F-M- strain. By counterimmunoelectrophoresis of the acid-extracted preparations with immune sera, all three strains were found to share three major antigens, but a fourth antigen with a net positive charge was present only in the F+M+ and F+M- strains. Antisera to five C. jejuni and two Campylobacter fetus strains recognized the 63-kDa protein of purified F+M+ flagella in Western blots, demonsating that a common antigen is present, but enzyme-linked immunosorbent assay results that the sharing of this antigen among Campylobacter strains is variable.

Original languageEnglish (US)
Pages (from-to)47-52
Number of pages6
JournalInfection and immunity
Volume53
Issue number1
DOIs
StatePublished - 1986
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Antigenicity of Campylobacter jejuni flagella'. Together they form a unique fingerprint.

Cite this