Antineutrophil monoclonal antibody (1F12) alters superoxide anion release by neutrophils and Kupffer cells

A. P. Bautista, Z. Spolarics, H. Jaeschke, C. W. Smith, J. J. Spitzer

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The formation of oxygen-derived radicals by phagocytes is regulated by chemotactic agents, cytokines, and adhesion molecules, such as CD11b/CD18 (Mac-1). In the rat system, we investigated the effect of monoclonal antibody 1F12 against rat neutrophils on hepatic sequestration of neutrophils and superoxide release by hepatic phagocytes. Within 15 min after 1F12 injection, there was profound neutropenia, which persisted for 24 h. The majority of the 'lost' neutrophils were sequestered in the liver 4 h after treatment. Zymosan-induced superoxide release in vitro by isolated hepatic neutrophils from 1F12-treated rats was significantly attenuated at 4 and 24 h. The phorbol myristate acetate mediated superoxide release was inhibited 24 h after treatment. Superoxide anion release by normal adherent neutrophils in the presence of agonists was also inhibited by 1F12 in vitro. The in vivo administration of 1F12 primed the Kupffer cells to release superoxide. In vitro treatment of Kupffer cells with 1F12 also stimulated superoxide release. Monoclonal antibody WT.3 (also directed against rat neutrophils), which does not cause neutropenia, did not alter superoxide generation by neutrophils and Kupffer cells. These results indicate that 1F12 may be useful in attenuating inflammation and tissue injury associated with neutrophil activation. However, the activation of Kupffer cells to release toxic oxygen- derived metabolites may predispose the liver to injury in certain pathological conditions.

Original languageEnglish (US)
Pages (from-to)328-335
Number of pages8
JournalJournal of Leukocyte Biology
Volume55
Issue number3
DOIs
StatePublished - Jan 1 1994

Fingerprint

Kupffer Cells
Superoxides
Neutrophils
Monoclonal Antibodies
Liver
Phagocytes
Neutropenia
Neutrophil Activation
Zymosan
Poisons
Wounds and Injuries
Tetradecanoylphorbol Acetate
Chemokines
Reactive Oxygen Species
Therapeutics
Oxygen
Inflammation
Injections
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Cell Biology

Keywords

  • activated macrophages
  • apoptosis
  • inflammation
  • liver
  • rat

Cite this

Bautista, A. P. ; Spolarics, Z. ; Jaeschke, H. ; Smith, C. W. ; Spitzer, J. J. / Antineutrophil monoclonal antibody (1F12) alters superoxide anion release by neutrophils and Kupffer cells. In: Journal of Leukocyte Biology. 1994 ; Vol. 55, No. 3. pp. 328-335.
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Antineutrophil monoclonal antibody (1F12) alters superoxide anion release by neutrophils and Kupffer cells. / Bautista, A. P.; Spolarics, Z.; Jaeschke, H.; Smith, C. W.; Spitzer, J. J.

In: Journal of Leukocyte Biology, Vol. 55, No. 3, 01.01.1994, p. 328-335.

Research output: Contribution to journalArticle

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