Antiplasmodial activity of the ethnobotanical plant Cassia fistula

Mary H. Grace, Carmen Lategan, Rocky Graziose, Peter J. Smith, Ilya Raskin, Mary Ann Lila

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

In our ongoing investigation of new compounds with activity against malaria parasites, we tested the in vitro antiplasmodial activity of fractions and purified compounds from Cassia fistula L., a plant traditionally used by native populations of Tanzania, Zimbabwe, Mozambique and Brazil to treat malaria or symptoms associated with this disease. Crude extracts from leaves, bark and fruits were tested for their antiplasmodial activity against the chloroquine-sensitive strain of Plasmodium falciparum (D10), where leaf extracts showed the highest activity. The chloroform extract of the leaves was further bioassay-guided fractionated using a combination of centrifugal partition chromatography and flash column chromatography. Three main antiplasmodial principles, phytol (1) (IC50 18.9 ± 0.60 μM), lutein (2) (IC50 12.5 ± 0.35 μM), and di-lineolylgalactopyranosyl- glycerol (DLGG) (IC50 5.8 ± 0.27 μM) (3), were isolated and identified using spectroscopic methods. When the three active principles were tested for their cytotoxicity using a Chinese Hamster Ovarian (CHO) cell line, compound 3 showed very weak toxicity (IC50 75.9 ± 0.28 μM), while the other two compounds were nontoxic, even at the highest concentration tested. The study provides evidence to support the use of Cassia fistula as an antimalarial remedy and describes the antiplasmodial constituents from the leaves.

Original languageEnglish (US)
Pages (from-to)1263-1266
Number of pages4
JournalNatural Product Communications
Volume7
Issue number10
DOIs
StatePublished - 2012

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine

Keywords

  • Antiplasmodial
  • Cassia fistula
  • Cytotoxicity
  • Glycolipid
  • Lutein
  • Phytol

Fingerprint Dive into the research topics of 'Antiplasmodial activity of the ethnobotanical plant Cassia fistula'. Together they form a unique fingerprint.

Cite this