Antisense intergenic transcription in V(D)J recombination

Daniel J. Bolland, Andrew L. Wood, Colette M. Johnston, Samuel Bunting, Geoff Morgan, Lyubomira Chakalova, Peter J. Fraser, Anne E. Corcoran

Research output: Contribution to journalArticle

178 Citations (Scopus)

Abstract

Antigen receptor genes undergo variable, diversity and joining (V(D)J) recombination, which requires ordered large-scale chromatin remodeling. Here we show that antisense transcription, both genic and intergenic, occurs extensively in the V region of the immunoglobulin heavy chain locus. RNA fluorescence in situ hybridization demonstrates antisense transcription is strictly developmentally regulated and is initiated during the transition from DJH to VDJH recombination and terminates concomitantly with VDJH recombination. Our data show antisense transcription is specific to the V region and suggest transcripts extend across several genes. We propose that antisense transcription remodels the V region to facilitate VH-to-DJH recombination. These findings have wider implications for V(D)J recombination of other antigen receptor loci and developmental regulation of multigene loci.

Original languageEnglish (US)
Pages (from-to)630-637
Number of pages8
JournalNature Immunology
Volume5
Issue number6
DOIs
StatePublished - Jun 1 2004
Externally publishedYes

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Genetic Recombination
Antigen Receptors
Immunoglobulin Heavy Chains
Chromatin Assembly and Disassembly
Fluorescence In Situ Hybridization
Genes
RNA

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Bolland, D. J., Wood, A. L., Johnston, C. M., Bunting, S., Morgan, G., Chakalova, L., ... Corcoran, A. E. (2004). Antisense intergenic transcription in V(D)J recombination. Nature Immunology, 5(6), 630-637. https://doi.org/10.1038/ni1068
Bolland, Daniel J. ; Wood, Andrew L. ; Johnston, Colette M. ; Bunting, Samuel ; Morgan, Geoff ; Chakalova, Lyubomira ; Fraser, Peter J. ; Corcoran, Anne E. / Antisense intergenic transcription in V(D)J recombination. In: Nature Immunology. 2004 ; Vol. 5, No. 6. pp. 630-637.
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Bolland, DJ, Wood, AL, Johnston, CM, Bunting, S, Morgan, G, Chakalova, L, Fraser, PJ & Corcoran, AE 2004, 'Antisense intergenic transcription in V(D)J recombination', Nature Immunology, vol. 5, no. 6, pp. 630-637. https://doi.org/10.1038/ni1068

Antisense intergenic transcription in V(D)J recombination. / Bolland, Daniel J.; Wood, Andrew L.; Johnston, Colette M.; Bunting, Samuel; Morgan, Geoff; Chakalova, Lyubomira; Fraser, Peter J.; Corcoran, Anne E.

In: Nature Immunology, Vol. 5, No. 6, 01.06.2004, p. 630-637.

Research output: Contribution to journalArticle

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Bolland DJ, Wood AL, Johnston CM, Bunting S, Morgan G, Chakalova L et al. Antisense intergenic transcription in V(D)J recombination. Nature Immunology. 2004 Jun 1;5(6):630-637. https://doi.org/10.1038/ni1068