Apoptotic death following Fas activation in human oligodendrocyte hybrid cultures

Weiping Li, Yasuhiro Maeda, Xue Ming, Stuart Cook, John Chapin, Walter Husar, Peter Dowling

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The purpose of this study was to determine how oligodendrocytes die following Fas receptor activation. An immortalized human oligodendrocyte hybrid line (MO3.13) was challenged with Fas ligand (FasL), and cell death was assessed by flow cytometry and DNA gel electrophoresis. Caspase activation was determined by either Western immunoblotting on cell extracts or by whole-cell flow cytometry. FasL challenge clearly induced substantial apoptotic cell death in the oligodendrocyte hybrid cell line, as judged by flow cytometry and by the presence of prominent low molecular weight DNA banding patterns after gel electrophoresis. Western immunoblots showed marked increases in cleaved caspase-1, 8, and 3, indicating that the extrinsic Fas death receptor-induced pathway was activated. The intrinsic mitochondrial pathway was also activated, but only at a minimal level. These findings demonstrate that there are several independent molecular sites within the extrinsic caspase cascade in oligodendrocytes where inhibitory compounds may be capable of blocking cell death in vivo.

Original languageEnglish (US)
Pages (from-to)189-196
Number of pages8
JournalJournal of Neuroscience Research
Volume69
Issue number2
DOIs
StatePublished - Jul 15 2002

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Oligodendroglia
CD95 Antigens
Flow Cytometry
Fas Ligand Protein
Cell Death
Caspases
Electrophoresis
Western Blotting
Gels
Caspase 1
Death Domain Receptors
Caspase 8
Hybrid Cells
DNA
Cell Extracts
Caspase 3
Molecular Weight
Cell Line

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Cite this

Li, Weiping ; Maeda, Yasuhiro ; Ming, Xue ; Cook, Stuart ; Chapin, John ; Husar, Walter ; Dowling, Peter. / Apoptotic death following Fas activation in human oligodendrocyte hybrid cultures. In: Journal of Neuroscience Research. 2002 ; Vol. 69, No. 2. pp. 189-196.
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Apoptotic death following Fas activation in human oligodendrocyte hybrid cultures. / Li, Weiping; Maeda, Yasuhiro; Ming, Xue; Cook, Stuart; Chapin, John; Husar, Walter; Dowling, Peter.

In: Journal of Neuroscience Research, Vol. 69, No. 2, 15.07.2002, p. 189-196.

Research output: Contribution to journalArticle

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