Application of isozyme electrophoresis for purity testing and cultivar identification of F1 hybrids of Brassica oleracea

P. Arús, C. R. Shields, T. J. Orton

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Six isozyme genes were analyzed in seed samples of 65 commercial F1 hybrids of four horticultural groups of Brassica oleracea (cabbage, Brussels sprouts, sprouting broccoli and cauliflower). Results obtained from electrophoretic assays led to the following conclusions: 1) the electrophoretic test of F1 hybrid purity was possible in 59 (91%) of the hybrids analyzed, since their inbred parents were apparently fixed each for a different allele in at least on of the loci studied; 2) forty-eight (74%) of the hybrids were individually distinguished by their isozyme phenotype; 3) high levels of segregation in the inbred parents were inferred from the analysis of a sample of seeds of each hybrid.

Original languageEnglish (US)
Pages (from-to)651-657
Number of pages7
JournalEuphytica
Volume34
Issue number3
DOIs
StatePublished - Nov 1985
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture

Keywords

  • Brassica oleracea
  • Brussels sprouts
  • cabbage
  • cauliflower
  • cultivar identification
  • isozymes, F hybrid purity testing
  • sprouting broccoli

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