Arabidopsis γ-glutamylcyclotransferase affects glutathione content and root system architecture during sulfur starvation

Naveen C. Joshi, Andreas J. Meyer, Sajid A.K. Bangash, Zhi Liang Zheng, Thomas Leustek

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

γ-Glutamylcyclotransferase initiates glutathione degradation to component amino acids l-glutamate, l-cysteine and l-glycine. The enzyme is encoded by three genes in Arabidopsis thaliana, one of which (GGCT2;1) is transcriptionally upregulated by starvation for the essential macronutrient sulfur (S). Regulation by S-starvation suggests that GGCT2;1 mobilizes l-cysteine from glutathione when there is insufficient sulfate for de novo l-cysteine synthesis. The response of wild-type seedlings to S-starvation was compared to ggct2;1 null mutants. S-starvation causes glutathione depletion in S-starved wild-type seedlings, but higher glutathione is maintained in the primary root tip than in other seedling tissues. Although GGCT2;1 is induced throughout seedlings, its expression is concentrated in the primary root tip where it activates the γ-glutamyl cycle. S-starved wild-type plants also produce longer primary roots, and lateral root growth is suppressed. While glutathione is also rapidly depleted in ggct2;1 null seedlings, much higher glutathione is maintained in the primary root tip compared to the wild-type. S-starved ggct2;1 primary roots grow longer than the wild-type, and lateral root growth is not suppressed. These results point to a role for GGCT2;1 in S-starvation-response changes to root system architecture through activity of the γ-glutamyl cycle in the primary root tip. l-Cysteine mobilization from glutathione is not solely a function of GGCT2;1.

Original languageEnglish (US)
Pages (from-to)1387-1397
Number of pages11
JournalNew Phytologist
Volume221
Issue number3
DOIs
StatePublished - Feb 1 2019

Fingerprint

Starvation
Sulfur
Arabidopsis
Glutathione
starvation
root systems
glutathione
sulfur
Seedlings
Meristem
root tips
Cysteine
cysteine
seedlings
root growth
Activity Cycles
Growth
glycine (amino acid)
glutamates
Glycine

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Keywords

  • cysteine
  • glutathione
  • nutrient foraging
  • root system architecture
  • sulfur (S)
  • γ-glutamyl-cyclotransferase

Cite this

Joshi, Naveen C. ; Meyer, Andreas J. ; Bangash, Sajid A.K. ; Zheng, Zhi Liang ; Leustek, Thomas. / Arabidopsis γ-glutamylcyclotransferase affects glutathione content and root system architecture during sulfur starvation. In: New Phytologist. 2019 ; Vol. 221, No. 3. pp. 1387-1397.
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Arabidopsis γ-glutamylcyclotransferase affects glutathione content and root system architecture during sulfur starvation. / Joshi, Naveen C.; Meyer, Andreas J.; Bangash, Sajid A.K.; Zheng, Zhi Liang; Leustek, Thomas.

In: New Phytologist, Vol. 221, No. 3, 01.02.2019, p. 1387-1397.

Research output: Contribution to journalArticle

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T1 - Arabidopsis γ-glutamylcyclotransferase affects glutathione content and root system architecture during sulfur starvation

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AU - Meyer, Andreas J.

AU - Bangash, Sajid A.K.

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