Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling

Zengxiang Ge, Tabata Bergonci, Yuling Zhao, Yanjiao Zou, Shuo Du, Ming Che Liu, Xingju Luo, Hao Ruan, Liliana E. García-Valencia, Sheng Zhong, Saiying Hou, Qingpei Huang, Luhua Lai, Daniel S. Moura, Hongya Gu, Juan Dong, Hen Ming Wu, Thomas Dresselhaus, Junyu Xiao, Alice Y. CheungLi Jia Qu

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

In flowering plants, fertilization requires complex cell-to-cell communication events between the pollen tube and the female reproductive tissues, which are controlled by extracellular signaling molecules interacting with receptors at the pollen tube surface.We found that two such receptors in Arabidopsis, BUPS1 and BUPS2, and their peptide ligands, RALF4 and RALF19, are pollen tube-expressed and are required to maintain pollen tube integrity. BUPS1 and BUPS2 interact with receptors ANXUR1 and ANXUR2 via their ectodomains, and both sets of receptors bind RALF4 and RALF19. These receptor-ligand interactions are in competition with the female-derived ligand RALF34, which induces pollen tube bursting at nanomolar concentrations.We propose that RALF34 replaces RALF4 and RALF19 at the interface of pollen tube-female gametophyte contact, thereby deregulating BUPS-ANXUR signaling and in turn leading to pollen tube rupture and sperm release.

Original languageEnglish (US)
Pages (from-to)1596-1600
Number of pages5
JournalScience
Volume358
Issue number6370
DOIs
StatePublished - Dec 22 2017

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Pollen Tube
Arabidopsis
Spermatozoa
Ligands
Fertilization
Cell Communication
Rupture
Peptides

All Science Journal Classification (ASJC) codes

  • General

Cite this

Ge, Z., Bergonci, T., Zhao, Y., Zou, Y., Du, S., Liu, M. C., ... Qu, L. J. (2017). Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling. Science, 358(6370), 1596-1600. https://doi.org/10.1126/science.aao3642
Ge, Zengxiang ; Bergonci, Tabata ; Zhao, Yuling ; Zou, Yanjiao ; Du, Shuo ; Liu, Ming Che ; Luo, Xingju ; Ruan, Hao ; García-Valencia, Liliana E. ; Zhong, Sheng ; Hou, Saiying ; Huang, Qingpei ; Lai, Luhua ; Moura, Daniel S. ; Gu, Hongya ; Dong, Juan ; Wu, Hen Ming ; Dresselhaus, Thomas ; Xiao, Junyu ; Cheung, Alice Y. ; Qu, Li Jia. / Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling. In: Science. 2017 ; Vol. 358, No. 6370. pp. 1596-1600.
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title = "Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling",
abstract = "In flowering plants, fertilization requires complex cell-to-cell communication events between the pollen tube and the female reproductive tissues, which are controlled by extracellular signaling molecules interacting with receptors at the pollen tube surface.We found that two such receptors in Arabidopsis, BUPS1 and BUPS2, and their peptide ligands, RALF4 and RALF19, are pollen tube-expressed and are required to maintain pollen tube integrity. BUPS1 and BUPS2 interact with receptors ANXUR1 and ANXUR2 via their ectodomains, and both sets of receptors bind RALF4 and RALF19. These receptor-ligand interactions are in competition with the female-derived ligand RALF34, which induces pollen tube bursting at nanomolar concentrations.We propose that RALF34 replaces RALF4 and RALF19 at the interface of pollen tube-female gametophyte contact, thereby deregulating BUPS-ANXUR signaling and in turn leading to pollen tube rupture and sperm release.",
author = "Zengxiang Ge and Tabata Bergonci and Yuling Zhao and Yanjiao Zou and Shuo Du and Liu, {Ming Che} and Xingju Luo and Hao Ruan and Garc{\'i}a-Valencia, {Liliana E.} and Sheng Zhong and Saiying Hou and Qingpei Huang and Luhua Lai and Moura, {Daniel S.} and Hongya Gu and Juan Dong and Wu, {Hen Ming} and Thomas Dresselhaus and Junyu Xiao and Cheung, {Alice Y.} and Qu, {Li Jia}",
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Ge, Z, Bergonci, T, Zhao, Y, Zou, Y, Du, S, Liu, MC, Luo, X, Ruan, H, García-Valencia, LE, Zhong, S, Hou, S, Huang, Q, Lai, L, Moura, DS, Gu, H, Dong, J, Wu, HM, Dresselhaus, T, Xiao, J, Cheung, AY & Qu, LJ 2017, 'Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling', Science, vol. 358, no. 6370, pp. 1596-1600. https://doi.org/10.1126/science.aao3642

Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling. / Ge, Zengxiang; Bergonci, Tabata; Zhao, Yuling; Zou, Yanjiao; Du, Shuo; Liu, Ming Che; Luo, Xingju; Ruan, Hao; García-Valencia, Liliana E.; Zhong, Sheng; Hou, Saiying; Huang, Qingpei; Lai, Luhua; Moura, Daniel S.; Gu, Hongya; Dong, Juan; Wu, Hen Ming; Dresselhaus, Thomas; Xiao, Junyu; Cheung, Alice Y.; Qu, Li Jia.

In: Science, Vol. 358, No. 6370, 22.12.2017, p. 1596-1600.

Research output: Contribution to journalArticle

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T1 - Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling

AU - Ge, Zengxiang

AU - Bergonci, Tabata

AU - Zhao, Yuling

AU - Zou, Yanjiao

AU - Du, Shuo

AU - Liu, Ming Che

AU - Luo, Xingju

AU - Ruan, Hao

AU - García-Valencia, Liliana E.

AU - Zhong, Sheng

AU - Hou, Saiying

AU - Huang, Qingpei

AU - Lai, Luhua

AU - Moura, Daniel S.

AU - Gu, Hongya

AU - Dong, Juan

AU - Wu, Hen Ming

AU - Dresselhaus, Thomas

AU - Xiao, Junyu

AU - Cheung, Alice Y.

AU - Qu, Li Jia

PY - 2017/12/22

Y1 - 2017/12/22

N2 - In flowering plants, fertilization requires complex cell-to-cell communication events between the pollen tube and the female reproductive tissues, which are controlled by extracellular signaling molecules interacting with receptors at the pollen tube surface.We found that two such receptors in Arabidopsis, BUPS1 and BUPS2, and their peptide ligands, RALF4 and RALF19, are pollen tube-expressed and are required to maintain pollen tube integrity. BUPS1 and BUPS2 interact with receptors ANXUR1 and ANXUR2 via their ectodomains, and both sets of receptors bind RALF4 and RALF19. These receptor-ligand interactions are in competition with the female-derived ligand RALF34, which induces pollen tube bursting at nanomolar concentrations.We propose that RALF34 replaces RALF4 and RALF19 at the interface of pollen tube-female gametophyte contact, thereby deregulating BUPS-ANXUR signaling and in turn leading to pollen tube rupture and sperm release.

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