TY - JOUR
T1 - Arsenate respiratory reductase gene (arrA) for Desulfosporosinus sp. strain Y5
AU - Pérez-Jiménez, José R.
AU - DeFraia, Christopher
AU - Young, L. Y.
N1 - Funding Information:
This work was supported in part by NIEHS (#N00014-99-1-0), NSF CHE-0221978 (CEBIC), and by a CEBIC Summer Fellowship to C.D. We are grateful to Elizabeth García-Domínguez, Amber Pascal, Ronald S. Oremland, Elizabeth D. Rhine, John Scrivo, and Jodi Switzer Blum for cultures provided of Desulfosporosinus sp. strain Y5, E. coli , P. putida , and B. selenitireducens . We would also like to thank Dr. David J. Scala for helpful discussions and review of the manuscript.
PY - 2005/12/16
Y1 - 2005/12/16
N2 - Desulfosporosinus sp. strain Y5 is a spore-forming bacterium capable of dissimilatory arsenate reduction coupled to the oxidation of aromatic compounds. In arsenate respiration, the arsenate respiratory reductase (ARR) catalyzes the reduction of arsenate to arsenite. Our objective is to characterize the arrA gene, encoding the ARR, for Desulfosporosinus sp. strain Y5. Oligonucleotide primers were designed based on the few arrA gene sequences available at the time and validated against positive and negative controls. The resulting arrA-amplicon of ∼2.0 kb was cloned and sequenced. The arrA from Desulfosporosinus sp. Y5 is closely related to Desulfitobacterium hafniense (similarity of 77% and 81% at the nucleotide and amino acid levels, respectively). Phylogenetic topology based on the arrA gene was partially congruent with that of 16S rRNA-based analysis. This arrA sequence will support the development of specific tracking probes for Desulfosporosinus sp. Y5 and the molecular characterization and monitoring of dissimilatory arsenate reducing bacteria.
AB - Desulfosporosinus sp. strain Y5 is a spore-forming bacterium capable of dissimilatory arsenate reduction coupled to the oxidation of aromatic compounds. In arsenate respiration, the arsenate respiratory reductase (ARR) catalyzes the reduction of arsenate to arsenite. Our objective is to characterize the arrA gene, encoding the ARR, for Desulfosporosinus sp. strain Y5. Oligonucleotide primers were designed based on the few arrA gene sequences available at the time and validated against positive and negative controls. The resulting arrA-amplicon of ∼2.0 kb was cloned and sequenced. The arrA from Desulfosporosinus sp. Y5 is closely related to Desulfitobacterium hafniense (similarity of 77% and 81% at the nucleotide and amino acid levels, respectively). Phylogenetic topology based on the arrA gene was partially congruent with that of 16S rRNA-based analysis. This arrA sequence will support the development of specific tracking probes for Desulfosporosinus sp. Y5 and the molecular characterization and monitoring of dissimilatory arsenate reducing bacteria.
KW - Arsenate respiration
KW - Arsenate respiratory reductase
KW - Desulfosporosinus sp. strain Y5
KW - Dissimilatory arsenate reducing prokaryotes
KW - Dissimilatory arsenate reduction
KW - arrA gene
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U2 - 10.1016/j.bbrc.2005.10.011
DO - 10.1016/j.bbrc.2005.10.011
M3 - Article
C2 - 16242665
AN - SCOPUS:27744451137
SN - 0006-291X
VL - 338
SP - 825
EP - 829
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -