Arsenate respiratory reductase gene (arrA) for Desulfosporosinus sp. strain Y5

José R. Pérez-Jiménez, Christopher DeFraia, L. Y. Young

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Desulfosporosinus sp. strain Y5 is a spore-forming bacterium capable of dissimilatory arsenate reduction coupled to the oxidation of aromatic compounds. In arsenate respiration, the arsenate respiratory reductase (ARR) catalyzes the reduction of arsenate to arsenite. Our objective is to characterize the arrA gene, encoding the ARR, for Desulfosporosinus sp. strain Y5. Oligonucleotide primers were designed based on the few arrA gene sequences available at the time and validated against positive and negative controls. The resulting arrA-amplicon of ∼2.0 kb was cloned and sequenced. The arrA from Desulfosporosinus sp. Y5 is closely related to Desulfitobacterium hafniense (similarity of 77% and 81% at the nucleotide and amino acid levels, respectively). Phylogenetic topology based on the arrA gene was partially congruent with that of 16S rRNA-based analysis. This arrA sequence will support the development of specific tracking probes for Desulfosporosinus sp. Y5 and the molecular characterization and monitoring of dissimilatory arsenate reducing bacteria.

Original languageEnglish (US)
Pages (from-to)825-829
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Dec 16 2005

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


  • Arsenate respiration
  • Arsenate respiratory reductase
  • Desulfosporosinus sp. strain Y5
  • Dissimilatory arsenate reducing prokaryotes
  • Dissimilatory arsenate reduction
  • arrA gene


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