Assembly of nanoparticles from bioactive peptides and chitosan

B. Hu, Qingrong Huang, X. X. Zeng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Assembly of nanoparticles from bioactive peptides, caseinophosphopeptides (CPPs) and chitosan (CS) at physiological conditions and various CS/CPPs mass ratios have been systematically studied using a combination of turbidimetric titration, dynamic light scattering (DLS), electrophoretic mobility (zeta-potential) and transmission electron microscopy (TEM). Peptides, incorporated with CS forming nanoparticles, have already been prepared and identified using liquid chromatography-tandem mass spectrometry (LC-MS-MS). They are characteristic with different amount of clusters of phosphorylated seryl residues. At low salt concentration, an increase of CS/CPP mass ratio shifted the critical pHφ1, which designated the formation of CS/CPP nanocomplexes, as well as pHmax, representing the neutralization of positive and negative charge to higher pH values. The peptide-polymer binding mechanism was analyzed according to the results of DLS, electrophoretic mobility, and TEM. First, negatively charged CPPs absorbed to positively charged CS molecular chain to form intrapolymer nanocomplexes saturated with CPPs (CPPNP). Then, the negatively charged CPPNP was bridged by added positively charged CS. Finally, novel nano-scaled spherical brushes were formed as additional CS molecule absorbed back to and bound the CPPNP. Phosphorylated groups in the CPPs might be the dominant sites for interaction with -NH 3+ on the CS molecular chain.

Original languageEnglish (US)
Title of host publicationPolymer-Based Materials and Composites - Synthesis, Assembly, Properties and Applications
Pages431-436
Number of pages6
DOIs
Publication statusPublished - Oct 3 2011
Event2010 MRS Fall Meeting - Boston, MA, United States
Duration: Nov 29 2010Dec 3 2010

Publication series

NameMaterials Research Society Symposium Proceedings
Volume1312
ISSN (Print)0272-9172

Other

Other2010 MRS Fall Meeting
CountryUnited States
CityBoston, MA
Period11/29/1012/3/10

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Hu, B., Huang, Q., & Zeng, X. X. (2011). Assembly of nanoparticles from bioactive peptides and chitosan. In Polymer-Based Materials and Composites - Synthesis, Assembly, Properties and Applications (pp. 431-436). (Materials Research Society Symposium Proceedings; Vol. 1312). https://doi.org/10.1557/opl.2011.205