Assessing Molecular Epidemiology of Carbapenem-resistant Klebsiella pneumoniae (CR-KP) with MLST and MALDI-TOF in Central China

Xiujuan Meng; Jun Yang; Juping Duan; Sidi Liu; Xun Huang; Ximao Wen; Xin Huang; Chenchao Fu; Jie Li; Qingya Dou; Yao Liu; Jia Wang; Qun Yan; Mingxiang Zou; Wenen Liu; Zhong Peng; Liang Chen; Chunhui Li; Anhua Wu

doi:10.1038/s41598-018-38295-8

Assessing Molecular Epidemiology of Carbapenem-resistant Klebsiella pneumoniae (CR-KP) with MLST and MALDI-TOF in Central China

Xiujuan Meng, Jun Yang, Juping Duan, Sidi Liu, Xun Huang, Ximao Wen, Xin Huang, Chenchao Fu, Jie Li, Qingya Dou, Yao Liu, Jia Wang, Qun Yan, Mingxiang Zou, Wenen Liu, Zhong Peng, Liang Chen, Chunhui Li, Anhua Wu

New Jersey Medical School (NJMS), Public Health Research Institute Center

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Carbapenem-resistant K. pneumoniae (CR-KP) posts significant public health challenge worldwide. The aim of this study is to assess clinical characteristics and molecular epidemiology of CR-KP infections with Multilocus sequence typing (MLST) and Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF) in Central China. A total of 71 CR-KP isolates were recovered in a teaching hospital from October 2014 to December 2015. Among all CR-KP isolates, 73.2% (52) produced K. pneumoniae carbapenemases-2 (KPC-2). Eighteen ST types were identified by MLST, among these ST types, forty-seven isolates belonged to ST11 type, which was the predominant outbreak strain in China, and most ST11 isolates produced KPC-2. Eleven mass spectrometry (MS) types were identified by MALDI-TOF MS analysis, 53.5% isolates were MS4 and MS6, which matched with ST11 in MLST analysis. CR-KP infection was associated with increased medical cost and longer hospitalization. Therefore, we found that KPC-2-producing ST11 (MS4 and MS6) CR-KP isolates were the predominant clone identified by MLST and MALDI-TOF, and CR-KP infection was associated with increased hospital costs and longer hospitalization.

Original language	English (US)
Article number	2271
Journal	Scientific reports
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-38295-8
State	Published - Dec 1 2019

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Meng, X., Yang, J., Duan, J., Liu, S., Huang, X., Wen, X., Huang, X., Fu, C., Li, J., Dou, Q., Liu, Y., Wang, J., Yan, Q., Zou, M., Liu, W., Peng, Z., Chen, L., Li, C., & Wu, A. (2019). Assessing Molecular Epidemiology of Carbapenem-resistant Klebsiella pneumoniae (CR-KP) with MLST and MALDI-TOF in Central China. Scientific reports, 9(1), Article 2271. https://doi.org/10.1038/s41598-018-38295-8

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title = "Assessing Molecular Epidemiology of Carbapenem-resistant Klebsiella pneumoniae (CR-KP) with MLST and MALDI-TOF in Central China",

abstract = "Carbapenem-resistant K. pneumoniae (CR-KP) posts significant public health challenge worldwide. The aim of this study is to assess clinical characteristics and molecular epidemiology of CR-KP infections with Multilocus sequence typing (MLST) and Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF) in Central China. A total of 71 CR-KP isolates were recovered in a teaching hospital from October 2014 to December 2015. Among all CR-KP isolates, 73.2% (52) produced K. pneumoniae carbapenemases-2 (KPC-2). Eighteen ST types were identified by MLST, among these ST types, forty-seven isolates belonged to ST11 type, which was the predominant outbreak strain in China, and most ST11 isolates produced KPC-2. Eleven mass spectrometry (MS) types were identified by MALDI-TOF MS analysis, 53.5% isolates were MS4 and MS6, which matched with ST11 in MLST analysis. CR-KP infection was associated with increased medical cost and longer hospitalization. Therefore, we found that KPC-2-producing ST11 (MS4 and MS6) CR-KP isolates were the predominant clone identified by MLST and MALDI-TOF, and CR-KP infection was associated with increased hospital costs and longer hospitalization.",

author = "Xiujuan Meng and Jun Yang and Juping Duan and Sidi Liu and Xun Huang and Ximao Wen and Xin Huang and Chenchao Fu and Jie Li and Qingya Dou and Yao Liu and Jia Wang and Qun Yan and Mingxiang Zou and Wenen Liu and Zhong Peng and Liang Chen and Chunhui Li and Anhua Wu",

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Meng, X, Yang, J, Duan, J, Liu, S, Huang, X, Wen, X, Huang, X, Fu, C, Li, J, Dou, Q, Liu, Y, Wang, J, Yan, Q, Zou, M, Liu, W, Peng, Z, Chen, L, Li, C & Wu, A 2019, 'Assessing Molecular Epidemiology of Carbapenem-resistant Klebsiella pneumoniae (CR-KP) with MLST and MALDI-TOF in Central China', Scientific reports, vol. 9, no. 1, 2271. https://doi.org/10.1038/s41598-018-38295-8

TY - JOUR

T1 - Assessing Molecular Epidemiology of Carbapenem-resistant Klebsiella pneumoniae (CR-KP) with MLST and MALDI-TOF in Central China

AU - Meng, Xiujuan

AU - Yang, Jun

AU - Duan, Juping

AU - Liu, Sidi

AU - Huang, Xun

AU - Wen, Ximao

AU - Huang, Xin

AU - Fu, Chenchao

AU - Li, Jie

AU - Dou, Qingya

AU - Liu, Yao

AU - Wang, Jia

AU - Yan, Qun

AU - Zou, Mingxiang

AU - Liu, Wenen

AU - Peng, Zhong

AU - Chen, Liang

AU - Li, Chunhui

AU - Wu, Anhua

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Carbapenem-resistant K. pneumoniae (CR-KP) posts significant public health challenge worldwide. The aim of this study is to assess clinical characteristics and molecular epidemiology of CR-KP infections with Multilocus sequence typing (MLST) and Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF) in Central China. A total of 71 CR-KP isolates were recovered in a teaching hospital from October 2014 to December 2015. Among all CR-KP isolates, 73.2% (52) produced K. pneumoniae carbapenemases-2 (KPC-2). Eighteen ST types were identified by MLST, among these ST types, forty-seven isolates belonged to ST11 type, which was the predominant outbreak strain in China, and most ST11 isolates produced KPC-2. Eleven mass spectrometry (MS) types were identified by MALDI-TOF MS analysis, 53.5% isolates were MS4 and MS6, which matched with ST11 in MLST analysis. CR-KP infection was associated with increased medical cost and longer hospitalization. Therefore, we found that KPC-2-producing ST11 (MS4 and MS6) CR-KP isolates were the predominant clone identified by MLST and MALDI-TOF, and CR-KP infection was associated with increased hospital costs and longer hospitalization.

AB - Carbapenem-resistant K. pneumoniae (CR-KP) posts significant public health challenge worldwide. The aim of this study is to assess clinical characteristics and molecular epidemiology of CR-KP infections with Multilocus sequence typing (MLST) and Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF) in Central China. A total of 71 CR-KP isolates were recovered in a teaching hospital from October 2014 to December 2015. Among all CR-KP isolates, 73.2% (52) produced K. pneumoniae carbapenemases-2 (KPC-2). Eighteen ST types were identified by MLST, among these ST types, forty-seven isolates belonged to ST11 type, which was the predominant outbreak strain in China, and most ST11 isolates produced KPC-2. Eleven mass spectrometry (MS) types were identified by MALDI-TOF MS analysis, 53.5% isolates were MS4 and MS6, which matched with ST11 in MLST analysis. CR-KP infection was associated with increased medical cost and longer hospitalization. Therefore, we found that KPC-2-producing ST11 (MS4 and MS6) CR-KP isolates were the predominant clone identified by MLST and MALDI-TOF, and CR-KP infection was associated with increased hospital costs and longer hospitalization.

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Assessing Molecular Epidemiology of Carbapenem-resistant Klebsiella pneumoniae (CR-KP) with MLST and MALDI-TOF in Central China

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