Assessment of region, farming system, irrigation source and sampling time as food safety risk factors for tomatoes

Sivaranjani Pagadala, Sasha C. Marine, Shirley A. Micallef, Fei Wang, Donna M. Pahl, Meredith V. Melendez, Wesley L. Kline, Ruth A. Oni, Christopher S. Walsh, Kathryne L. Everts, Robert L. Buchanan

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

In the mid-Atlantic region of the United States, small- and medium-sized farmers use varied farm management methods and water sources to produce tomatoes. It is unclear whether these practices affect the food safety risk for tomatoes. This study was conducted to determine the prevalence, and assess risk factors for Salmonella enterica, Shiga toxin-producing Escherichia coli (STEC) and bacterial indicators in pre-harvest tomatoes and their production areas. A total of 24 organic and conventional, small- to medium-sized farms were sampled for six weeks in Maryland (MD), Delaware (DE) and New Jersey (NJ) between July and September 2012, and analyzed for indicator bacteria, Salmonella and STEC. A total of 422 samples - tomato fruit, irrigation water, compost, field soil and pond sediment samples - were collected, 259 of which were tomato samples. A low level of Salmonella-specific invA and Shiga toxin genes (stx1 or stx2) were detected, but no Salmonella or STEC isolates were recovered. Of the 422 samples analyzed, 9.5% were positive for generic E. coli, found in 5.4% (n=259) of tomato fruits, 22.5% (n=102) of irrigation water, 8.9% (n=45) of soil, 3/9 of pond sediment and 0/7 of compost samples. For tomato fruit, farming system (organic versus conventional) was not a significant factor for levels of indicator bacteria. However, the total number of organic tomato samples positive for generic E. coli (1.6%; 2/129) was significantly lower than for conventional tomatoes (6.9% (9/130); (χ2 (1)=4.60, p=0.032)). Region was a significant factor for levels of Total Coliforms (TC) (p=0.046), although differences were marginal, with western MD having the highest TC counts (2.6logCFU/g) and NJ having the lowest (2.0logCFU/g). Tomatoes touching the ground or plastic mulch harbored significantly higher levels of TC compared to vine tomatoes, signaling a potential risk factor. Source of irrigation water was a significant factor for all indicator bacteria (p<0.0001), and groundwater had lower bacterial levels than surface water. End of line surface water samples were not significantly different from source water samples, but end of line groundwater samples had significantly higher bacterial counts than source (p<0.0001), suggesting that Good Agricultural Practices that focus on irrigation line maintenance might be beneficial. In general, local effects other than cropping practices, including topography, land use and adjacent industries, might be important factors contributing to microbiological inputs on small- and medium-sized farms in the mid-Atlantic region.

Original languageEnglish (US)
Pages (from-to)98-108
Number of pages11
JournalInternational journal of food microbiology
Volume196
DOIs
StatePublished - Mar 2 2015

Fingerprint

Food Safety
Lycopersicon esculentum
Agriculture
food safety
risk factors
farming systems
tomatoes
irrigation
Water
Shiga-Toxigenic Escherichia coli
Shiga toxin-producing Escherichia coli
sampling
Mid-Atlantic Region
Soil
Salmonella
indicator species
Mid-Atlantic region
irrigation water
Fruit
Groundwater

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology

Keywords

  • Conventional and organic
  • Food safety
  • Fresh market tomatoes
  • Irrigation water
  • Mid-Atlantic
  • Pre-harvest contamination

Cite this

Pagadala, Sivaranjani ; Marine, Sasha C. ; Micallef, Shirley A. ; Wang, Fei ; Pahl, Donna M. ; Melendez, Meredith V. ; Kline, Wesley L. ; Oni, Ruth A. ; Walsh, Christopher S. ; Everts, Kathryne L. ; Buchanan, Robert L. / Assessment of region, farming system, irrigation source and sampling time as food safety risk factors for tomatoes. In: International journal of food microbiology. 2015 ; Vol. 196. pp. 98-108.
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abstract = "In the mid-Atlantic region of the United States, small- and medium-sized farmers use varied farm management methods and water sources to produce tomatoes. It is unclear whether these practices affect the food safety risk for tomatoes. This study was conducted to determine the prevalence, and assess risk factors for Salmonella enterica, Shiga toxin-producing Escherichia coli (STEC) and bacterial indicators in pre-harvest tomatoes and their production areas. A total of 24 organic and conventional, small- to medium-sized farms were sampled for six weeks in Maryland (MD), Delaware (DE) and New Jersey (NJ) between July and September 2012, and analyzed for indicator bacteria, Salmonella and STEC. A total of 422 samples - tomato fruit, irrigation water, compost, field soil and pond sediment samples - were collected, 259 of which were tomato samples. A low level of Salmonella-specific invA and Shiga toxin genes (stx1 or stx2) were detected, but no Salmonella or STEC isolates were recovered. Of the 422 samples analyzed, 9.5{\%} were positive for generic E. coli, found in 5.4{\%} (n=259) of tomato fruits, 22.5{\%} (n=102) of irrigation water, 8.9{\%} (n=45) of soil, 3/9 of pond sediment and 0/7 of compost samples. For tomato fruit, farming system (organic versus conventional) was not a significant factor for levels of indicator bacteria. However, the total number of organic tomato samples positive for generic E. coli (1.6{\%}; 2/129) was significantly lower than for conventional tomatoes (6.9{\%} (9/130); (χ2 (1)=4.60, p=0.032)). Region was a significant factor for levels of Total Coliforms (TC) (p=0.046), although differences were marginal, with western MD having the highest TC counts (2.6logCFU/g) and NJ having the lowest (2.0logCFU/g). Tomatoes touching the ground or plastic mulch harbored significantly higher levels of TC compared to vine tomatoes, signaling a potential risk factor. Source of irrigation water was a significant factor for all indicator bacteria (p<0.0001), and groundwater had lower bacterial levels than surface water. End of line surface water samples were not significantly different from source water samples, but end of line groundwater samples had significantly higher bacterial counts than source (p<0.0001), suggesting that Good Agricultural Practices that focus on irrigation line maintenance might be beneficial. In general, local effects other than cropping practices, including topography, land use and adjacent industries, might be important factors contributing to microbiological inputs on small- and medium-sized farms in the mid-Atlantic region.",
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Assessment of region, farming system, irrigation source and sampling time as food safety risk factors for tomatoes. / Pagadala, Sivaranjani; Marine, Sasha C.; Micallef, Shirley A.; Wang, Fei; Pahl, Donna M.; Melendez, Meredith V.; Kline, Wesley L.; Oni, Ruth A.; Walsh, Christopher S.; Everts, Kathryne L.; Buchanan, Robert L.

In: International journal of food microbiology, Vol. 196, 02.03.2015, p. 98-108.

Research output: Contribution to journalArticle

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AU - Marine, Sasha C.

AU - Micallef, Shirley A.

AU - Wang, Fei

AU - Pahl, Donna M.

AU - Melendez, Meredith V.

AU - Kline, Wesley L.

AU - Oni, Ruth A.

AU - Walsh, Christopher S.

AU - Everts, Kathryne L.

AU - Buchanan, Robert L.

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