Assignment of a gene for adenosine deaminase to human chromosome 20

J. A. Tischfield; R. P. Creagan; E. A. Nichols; F. H. Ruddle

doi:10.1159/000152631

Assignment of a gene for adenosine deaminase to human chromosome 20

J. A. Tischfield, R. P. Creagan, E. A. Nichols, F. H. Ruddle

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57 Scopus citations

Abstract

Evidence, based on five series of mouse-human somatic cell hybrid clones, is presented for assignment of a human gene for adenosine deaminase (ADA: EC 3.5.4.4) to chromosome 20. The human form of adenosine deaminase expressed by the hybrid cells apearcd to be identical to the isozyme found in erythrocytes, judging from its electro-phoretic mobility and reactions with p-chloromercuribenzoic acid (PCMB). Absence of ADA activity has been associated with a combined immunodeficiency syndrome. The relationship of our data to hypotheses concerning this association is discussed.

Original language	English (US)
Pages (from-to)	1-11
Number of pages	11
Journal	Human Heredity
Volume	24
Issue number	1
DOIs	https://doi.org/10.1159/000152631
State	Published - Jan 1 1974
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics
Genetics(clinical)

Keywords

ADA
Gene assignment
Human Chromosome 20
Somatic cell hybridization

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10.1159/000152631

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abstract = "Evidence, based on five series of mouse-human somatic cell hybrid clones, is presented for assignment of a human gene for adenosine deaminase (ADA: EC 3.5.4.4) to chromosome 20. The human form of adenosine deaminase expressed by the hybrid cells apearcd to be identical to the isozyme found in erythrocytes, judging from its electro-phoretic mobility and reactions with p-chloromercuribenzoic acid (PCMB). Absence of ADA activity has been associated with a combined immunodeficiency syndrome. The relationship of our data to hypotheses concerning this association is discussed.",

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AU - Tischfield, J. A.

AU - Creagan, R. P.

AU - Nichols, E. A.

AU - Ruddle, F. H.

PY - 1974/1/1

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N2 - Evidence, based on five series of mouse-human somatic cell hybrid clones, is presented for assignment of a human gene for adenosine deaminase (ADA: EC 3.5.4.4) to chromosome 20. The human form of adenosine deaminase expressed by the hybrid cells apearcd to be identical to the isozyme found in erythrocytes, judging from its electro-phoretic mobility and reactions with p-chloromercuribenzoic acid (PCMB). Absence of ADA activity has been associated with a combined immunodeficiency syndrome. The relationship of our data to hypotheses concerning this association is discussed.

AB - Evidence, based on five series of mouse-human somatic cell hybrid clones, is presented for assignment of a human gene for adenosine deaminase (ADA: EC 3.5.4.4) to chromosome 20. The human form of adenosine deaminase expressed by the hybrid cells apearcd to be identical to the isozyme found in erythrocytes, judging from its electro-phoretic mobility and reactions with p-chloromercuribenzoic acid (PCMB). Absence of ADA activity has been associated with a combined immunodeficiency syndrome. The relationship of our data to hypotheses concerning this association is discussed.

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