TY - JOUR
T1 - Association of c‐myc protein with enzymes of DNA replication in high molecular weight fractions from mammalian cells
AU - Studzinski, George P.
AU - Shankavaram, Uma T.
AU - Moore, Dorothy C.
AU - Reddy, Prem Veer
PY - 1991/6
Y1 - 1991/6
N2 - Oncoprotein c‐myc is expressed in proliferating but not quiescent mammalian cells, and its overexpression or inappropriate expression is associated with malignant transformation. However, in spite of an intense interest, the normal function of this protein has remained elusive. As a step towards the elucidation of the function of c‐myc protein, we studied its distribution within several types of cells, including HL 60, K 562, COLO 320, and CHEF/18 cells. In all of the cells studied, c‐myc protein was detected in high molecular weight protein fractions, in 350–600 Kd range, in gel‐exclusion chromatography and sucrose gradient centrifugation. This distribution of c‐myc protein coincided with the distribution of DNA polymerase α and several other enzymes necessary for DNA replication. The data suggest that c‐myc product may be a component of the replitase complex of enzymes involved in nuclear DNA replication.
AB - Oncoprotein c‐myc is expressed in proliferating but not quiescent mammalian cells, and its overexpression or inappropriate expression is associated with malignant transformation. However, in spite of an intense interest, the normal function of this protein has remained elusive. As a step towards the elucidation of the function of c‐myc protein, we studied its distribution within several types of cells, including HL 60, K 562, COLO 320, and CHEF/18 cells. In all of the cells studied, c‐myc protein was detected in high molecular weight protein fractions, in 350–600 Kd range, in gel‐exclusion chromatography and sucrose gradient centrifugation. This distribution of c‐myc protein coincided with the distribution of DNA polymerase α and several other enzymes necessary for DNA replication. The data suggest that c‐myc product may be a component of the replitase complex of enzymes involved in nuclear DNA replication.
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U2 - 10.1002/jcp.1041470305
DO - 10.1002/jcp.1041470305
M3 - Article
C2 - 1648564
AN - SCOPUS:0025833311
SN - 0021-9541
VL - 147
SP - 412
EP - 419
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
IS - 3
ER -