Abstract
The effect of anisotonic NaCl treatment on fixation and repair of radiation-induced potentially lethal damage (PLD) was tested in normal human cells and in three homzygous ataxia-telangiectasia (A-T) and tow heterozygous A-T cell strains. Fixation of radiation-induced PLD occurred in all cell strains exposed to 0.05, 0.5, or 1.5 mole/liter NaCl solutions immediately after irradiation. This effect was observed in both plateau-phase and exponentially growing normal and A-T cells. When an incubation period at 37° C was introduced between irradiation and the subsequent anisotonic treatment, recovery was observed in both normal and A-T cells strains. These data show that A-T cells are as proficient as normal cells in repairing PLD that is sensitive to anisotonic NaCl treatment. It is proposed that two PLD repair systems may exist, one that is expressed after irradiation in proliferatively arrested cells and another that occurs in plateau-phase as well as exponentially growing cells, and is expressed by the postirradiation treatments described here and by Raaphorst and Azzam (Radiat. Res. 86, 52-66 (1981)).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 497-507 |
| Number of pages | 11 |
| Journal | Radiation Research |
| Volume | 101 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 1 1985 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Radiation
- Radiology Nuclear Medicine and imaging
Cite this
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Ataxia-telangiectasia homo- and heterozygous cells show a normal repair and fixation response to anisotonic NaCl treatment after irradiation. / Raaphorst, G. P.; Azzam, Edouard.
In: Radiation Research, Vol. 101, No. 3, 01.01.1985, p. 497-507.Research output: Contribution to journal › Article
TY - JOUR
T1 - Ataxia-telangiectasia homo- and heterozygous cells show a normal repair and fixation response to anisotonic NaCl treatment after irradiation
AU - Raaphorst, G. P.
AU - Azzam, Edouard
PY - 1985/1/1
Y1 - 1985/1/1
N2 - The effect of anisotonic NaCl treatment on fixation and repair of radiation-induced potentially lethal damage (PLD) was tested in normal human cells and in three homzygous ataxia-telangiectasia (A-T) and tow heterozygous A-T cell strains. Fixation of radiation-induced PLD occurred in all cell strains exposed to 0.05, 0.5, or 1.5 mole/liter NaCl solutions immediately after irradiation. This effect was observed in both plateau-phase and exponentially growing normal and A-T cells. When an incubation period at 37° C was introduced between irradiation and the subsequent anisotonic treatment, recovery was observed in both normal and A-T cells strains. These data show that A-T cells are as proficient as normal cells in repairing PLD that is sensitive to anisotonic NaCl treatment. It is proposed that two PLD repair systems may exist, one that is expressed after irradiation in proliferatively arrested cells and another that occurs in plateau-phase as well as exponentially growing cells, and is expressed by the postirradiation treatments described here and by Raaphorst and Azzam (Radiat. Res. 86, 52-66 (1981)).
AB - The effect of anisotonic NaCl treatment on fixation and repair of radiation-induced potentially lethal damage (PLD) was tested in normal human cells and in three homzygous ataxia-telangiectasia (A-T) and tow heterozygous A-T cell strains. Fixation of radiation-induced PLD occurred in all cell strains exposed to 0.05, 0.5, or 1.5 mole/liter NaCl solutions immediately after irradiation. This effect was observed in both plateau-phase and exponentially growing normal and A-T cells. When an incubation period at 37° C was introduced between irradiation and the subsequent anisotonic treatment, recovery was observed in both normal and A-T cells strains. These data show that A-T cells are as proficient as normal cells in repairing PLD that is sensitive to anisotonic NaCl treatment. It is proposed that two PLD repair systems may exist, one that is expressed after irradiation in proliferatively arrested cells and another that occurs in plateau-phase as well as exponentially growing cells, and is expressed by the postirradiation treatments described here and by Raaphorst and Azzam (Radiat. Res. 86, 52-66 (1981)).
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UR - http://www.scopus.com/inward/citedby.url?scp=0021979142&partnerID=8YFLogxK
U2 - 10.2307/3576509
DO - 10.2307/3576509
M3 - Article
C2 - 3983365
AN - SCOPUS:0021979142
VL - 101
SP - 497
EP - 507
JO - Radiation Research
JF - Radiation Research
SN - 0033-7587
IS - 3
ER -