Autoluminescent plants

Alexander Krichevsky, Benjamin Meyers, Alexander Vainstein, Pal Maliga, Vitaly Citovsky

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Prospects of obtaining plants glowing in the dark have captivated the imagination of scientists and layman alike. While light emission has been developed into a useful marker of gene expression, bioluminescence in plants remained dependent on externally supplied substrate. Evolutionary conservation of the prokaryotic gene expression machinery enabled expression of the six genes of the lux operon in chloroplasts yielding plants that are capable of autonomous light emission. This work demonstrates that complex metabolic pathways of prokaryotes can be reconstructed and function in plant chloroplasts and that transplastomic plants can emit light that is visible by naked eye.

Original languageEnglish (US)
Article numbere15461
JournalPloS one
Volume5
Issue number11
DOIs
StatePublished - Dec 9 2010

Fingerprint

Light emission
Gene expression
Bioluminescence
Machinery
Chloroplasts
Conservation
Gene Expression
Light
Genes
chloroplasts
Substrates
Imagination
gene expression
bioluminescence
operon
Operon
prokaryotic cells
Metabolic Networks and Pathways
biochemical pathways
eyes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Krichevsky, A., Meyers, B., Vainstein, A., Maliga, P., & Citovsky, V. (2010). Autoluminescent plants. PloS one, 5(11), [e15461]. https://doi.org/10.1371/journal.pone.0015461
Krichevsky, Alexander ; Meyers, Benjamin ; Vainstein, Alexander ; Maliga, Pal ; Citovsky, Vitaly. / Autoluminescent plants. In: PloS one. 2010 ; Vol. 5, No. 11.
@article{9cf399f467c746939b997ca9bbc62425,
title = "Autoluminescent plants",
abstract = "Prospects of obtaining plants glowing in the dark have captivated the imagination of scientists and layman alike. While light emission has been developed into a useful marker of gene expression, bioluminescence in plants remained dependent on externally supplied substrate. Evolutionary conservation of the prokaryotic gene expression machinery enabled expression of the six genes of the lux operon in chloroplasts yielding plants that are capable of autonomous light emission. This work demonstrates that complex metabolic pathways of prokaryotes can be reconstructed and function in plant chloroplasts and that transplastomic plants can emit light that is visible by naked eye.",
author = "Alexander Krichevsky and Benjamin Meyers and Alexander Vainstein and Pal Maliga and Vitaly Citovsky",
year = "2010",
month = "12",
day = "9",
doi = "10.1371/journal.pone.0015461",
language = "English (US)",
volume = "5",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",

}

Krichevsky, A, Meyers, B, Vainstein, A, Maliga, P & Citovsky, V 2010, 'Autoluminescent plants', PloS one, vol. 5, no. 11, e15461. https://doi.org/10.1371/journal.pone.0015461

Autoluminescent plants. / Krichevsky, Alexander; Meyers, Benjamin; Vainstein, Alexander; Maliga, Pal; Citovsky, Vitaly.

In: PloS one, Vol. 5, No. 11, e15461, 09.12.2010.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Autoluminescent plants

AU - Krichevsky, Alexander

AU - Meyers, Benjamin

AU - Vainstein, Alexander

AU - Maliga, Pal

AU - Citovsky, Vitaly

PY - 2010/12/9

Y1 - 2010/12/9

N2 - Prospects of obtaining plants glowing in the dark have captivated the imagination of scientists and layman alike. While light emission has been developed into a useful marker of gene expression, bioluminescence in plants remained dependent on externally supplied substrate. Evolutionary conservation of the prokaryotic gene expression machinery enabled expression of the six genes of the lux operon in chloroplasts yielding plants that are capable of autonomous light emission. This work demonstrates that complex metabolic pathways of prokaryotes can be reconstructed and function in plant chloroplasts and that transplastomic plants can emit light that is visible by naked eye.

AB - Prospects of obtaining plants glowing in the dark have captivated the imagination of scientists and layman alike. While light emission has been developed into a useful marker of gene expression, bioluminescence in plants remained dependent on externally supplied substrate. Evolutionary conservation of the prokaryotic gene expression machinery enabled expression of the six genes of the lux operon in chloroplasts yielding plants that are capable of autonomous light emission. This work demonstrates that complex metabolic pathways of prokaryotes can be reconstructed and function in plant chloroplasts and that transplastomic plants can emit light that is visible by naked eye.

UR - http://www.scopus.com/inward/record.url?scp=78649738138&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649738138&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0015461

DO - 10.1371/journal.pone.0015461

M3 - Article

C2 - 21103397

AN - SCOPUS:78649738138

VL - 5

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 11

M1 - e15461

ER -

Krichevsky A, Meyers B, Vainstein A, Maliga P, Citovsky V. Autoluminescent plants. PloS one. 2010 Dec 9;5(11). e15461. https://doi.org/10.1371/journal.pone.0015461