Autophagy prevents irradiation injury and maintains stemness through decreasing ROS generation in mesenchymal stem cells

J. Hou, Z. P. Han, Y. Y. Jing, X. Yang, S. S. Zhang, K. Sun, C. Hao, Y. Meng, F. H. Yu, X. Q. Liu, Y. F. Shi, M. C. Wu, L. Zhang, L. X. Wei

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

Stem cells were characterized by their stemness: self-renewal and pluripotency. Mesenchymal stem cells (MSCs) are a unique type of adult stem cells that have been proven to be involved in tissue repair, immunoloregulation and tumorigenesis. Irradiation is a well-known factor that leads to functional obstacle in stem cells. However, the mechanism of stemness maintenance in human MSCs exposed to irradiation remains unknown. We demonstrated that irradiation could induce reactive oxygen species (ROS) accumulation that resulted in DNA damage and stemness injury in MSCs. Autophagy induced by starvation or rapamycin can reduce ROS accumulation-associated DNA damage and maintain stemness in MSCs. Further, inhibition of autophagy leads to augment of ROS accumulation and DNA damage, which results in the loss of stemness in MSCs. Our results indicate that autophagy may have an important role in protecting stemness of MSCs from irradiation injury.

Original languageEnglish (US)
Article numbere844
JournalCell Death and Disease
Volume4
Issue number10
DOIs
StatePublished - Oct 1 2013
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Cancer Research

Keywords

  • Autophagy
  • Mesenchymal stem cells
  • Reactive oxygen species
  • Stemness

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    Hou, J., Han, Z. P., Jing, Y. Y., Yang, X., Zhang, S. S., Sun, K., Hao, C., Meng, Y., Yu, F. H., Liu, X. Q., Shi, Y. F., Wu, M. C., Zhang, L., & Wei, L. X. (2013). Autophagy prevents irradiation injury and maintains stemness through decreasing ROS generation in mesenchymal stem cells. Cell Death and Disease, 4(10), [e844]. https://doi.org/10.1038/cddis.2013.338