@inbook{438029f8d39543d9a7a52e725ba2f62e,
title = "Azide-Specific Labeling of Biomolecules by Staudinger–Bertozzi Ligation: Phosphine Derivatives of Fluorescent Probes Suitable for Single-Molecule Fluorescence Spectroscopy",
abstract = "We describe the synthesis of phosphine derivatives of three fluorescent probes that have a brightness and photostability suitable for single-molecule fluorescence spectroscopy and microscopy: Alexa488, Cy3B, and Alexa647. In addition, we describe procedures for use of these reagents in azide-specific, bioorthogonal labeling through Staudinger–Bertozzi ligation, as well as procedures for the quantitation of labeling specificity and labeling efficiency. The reagents and procedures of this report enable chemoselective, site-selective labeling of azide-containing biomolecules for single-molecule fluorescence spectroscopy and microscopy.",
author = "Anirban Chakraborty and Dongye Wang and Ebright, {Yon W.} and Ebright, {Richard H.}",
note = "Funding Information: We thank S. Weiss for suggesting identities of fluorescent probes suitable for single-molecule detection. This work was supported by NIH grants GM41376 and AI72766 and a Howard Hughes Investigatorship to R. H. E. Publisher Copyright: {\textcopyright} 2010 Elsevier Inc.",
year = "2010",
month = jan,
doi = "10.1016/S0076-6879(10)72018-8",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "19--30",
booktitle = "Methods in Enzymology",
address = "United States",
}