Bacterial DNA supercoiling and [ATP] [ADP]. Changes associated with a transition to anaerobic growth

Li Shan Hsieh, Richard M. Burger, Karl Drlica

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Shifting Escherichia coli from aerobic to anaerobic growth caused changes in the ratio of [ATP] [ADP] and in negative supercoiling of chromosomal and plasmid DNA. Shortly after lowering oxygen tension, both [ATP] [ADP] and supercoiling transiently decreased. Under conditions of exponential anaerobic growth, both were higher than under aerobic conditions. These correlations may reflect an effect of [ATP] [ADP] on DNA gyrase, since in vitro [ATP] [ADP] influences the level of plasmid supercoiling attained when gyrase is either introducing or removing supercoils. When the supercoiling activity of gyrase was perturbed by a mutation in gyrB, a shift to anaerobic conditions resulted in plasmid supercoil relaxation similar to that seen with wild-type. However, the low level of supercoiling in the mutant persisted during a time when supercoiling in wild-type recovered and then exceeded aerobic levels. Thus, changes in oxygen tension can alter DNA supercoiling through an effect on gyrase, and correlations exist between changes in supercoiling and changes in the intracellular ratio of [ATP] [ADP].

Original languageEnglish (US)
Pages (from-to)443-450
Number of pages8
JournalJournal of molecular biology
Volume219
Issue number3
DOIs
StatePublished - Jun 5 1991

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Bacterial DNA
Adenosine Diphosphate
Adenosine Triphosphate
Growth
Plasmids
DNA Gyrase
Oxygen
DNA
Escherichia coli
Mutation

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Keywords

  • ADP
  • ATP
  • DNA supercoiling
  • anaerobiosis
  • gyrase

Cite this

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title = "Bacterial DNA supercoiling and [ATP] [ADP]. Changes associated with a transition to anaerobic growth",
abstract = "Shifting Escherichia coli from aerobic to anaerobic growth caused changes in the ratio of [ATP] [ADP] and in negative supercoiling of chromosomal and plasmid DNA. Shortly after lowering oxygen tension, both [ATP] [ADP] and supercoiling transiently decreased. Under conditions of exponential anaerobic growth, both were higher than under aerobic conditions. These correlations may reflect an effect of [ATP] [ADP] on DNA gyrase, since in vitro [ATP] [ADP] influences the level of plasmid supercoiling attained when gyrase is either introducing or removing supercoils. When the supercoiling activity of gyrase was perturbed by a mutation in gyrB, a shift to anaerobic conditions resulted in plasmid supercoil relaxation similar to that seen with wild-type. However, the low level of supercoiling in the mutant persisted during a time when supercoiling in wild-type recovered and then exceeded aerobic levels. Thus, changes in oxygen tension can alter DNA supercoiling through an effect on gyrase, and correlations exist between changes in supercoiling and changes in the intracellular ratio of [ATP] [ADP].",
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Bacterial DNA supercoiling and [ATP] [ADP]. Changes associated with a transition to anaerobic growth. / Hsieh, Li Shan; Burger, Richard M.; Drlica, Karl.

In: Journal of molecular biology, Vol. 219, No. 3, 05.06.1991, p. 443-450.

Research output: Contribution to journalArticle

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