TY - JOUR
T1 - Bacterial DNA supercoiling and [ATP]/[ADP] ratio
T2 - Changes associated with salt shock
AU - Hsieh, L. S.
AU - Rouviere-Yaniv, J.
AU - Drlica, K.
PY - 1991
Y1 - 1991
N2 - When Escherichia coli K-12 was shifted from a medium lacking salt to one containing 0.5 M NaCl, both the [ATP]/[ADP] ratio and negative supercoiling of plasmid DNA increased within a few minutes. After about 10 min both declined, eventually reaching a level slightly above that observed with cells growing exponentially in the absence of salt. Since in vitro the [ATP]/[ADP] ratio influences the level of supercoiling generated by gyrase (H. Westerhoff, M. O'Dea, A. Maxwell, and M. Gellert, Cell Biophys. 12:157-181, 1988), the physiological response of supercoiling to salt shock is most easily explained by the sensitivity of gyrase to changes in the intracellular [ATP]/[ADP] ratio. This raises the possibility that the [ATP]/[ADP] ratio is an important factor in the control of supercoiling.
AB - When Escherichia coli K-12 was shifted from a medium lacking salt to one containing 0.5 M NaCl, both the [ATP]/[ADP] ratio and negative supercoiling of plasmid DNA increased within a few minutes. After about 10 min both declined, eventually reaching a level slightly above that observed with cells growing exponentially in the absence of salt. Since in vitro the [ATP]/[ADP] ratio influences the level of supercoiling generated by gyrase (H. Westerhoff, M. O'Dea, A. Maxwell, and M. Gellert, Cell Biophys. 12:157-181, 1988), the physiological response of supercoiling to salt shock is most easily explained by the sensitivity of gyrase to changes in the intracellular [ATP]/[ADP] ratio. This raises the possibility that the [ATP]/[ADP] ratio is an important factor in the control of supercoiling.
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U2 - 10.1128/jb.173.12.3914-3917.1991
DO - 10.1128/jb.173.12.3914-3917.1991
M3 - Comment/debate
C2 - 1646791
AN - SCOPUS:0025915548
SN - 0021-9193
VL - 173
SP - 3914
EP - 3917
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 12
ER -