Bacterial RNA polymerase subunit ω and eukaryotic polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly

Leonid Minakhin; Sechal Bhagat; Adrian Brunning; Elizabeth A. Campbell; Seth A. Darst; Richard H. Ebright; Konstantin Severinov

doi:10.1073/pnas.98.3.892

Bacterial RNA polymerase subunit ω and eukaryotic polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly

Leonid Minakhin, Sechal Bhagat, Adrian Brunning, Elizabeth A. Campbell, Seth A. Darst, Richard H. Ebright, Konstantin Severinov

Research output: Contribution to journal › Article › peer-review

156 Scopus citations

Abstract

Bacterial DNA-dependent RNA polymerase (RNAP) has subunit composition β′βα^Iα^II. The role of ω has been unclear. We show that is homologous in sequence and structure to RPB6, an essential subunit shared in eukaryotic RNAP I, II, and III. In Escherichia coli, overproduction of ω suppresses the assembly defect caused by substitution of residue 1362 of the largest subunit of RNAP,β′. In yeast, overproduction of RPB6 suppresses the assembly defect caused by the equivalent substitution in the largest subunit of RNAP II, RPB1. High-resolution structural analysis of the ω-β′ interface in bacterial RNAP, and comparison with the RPB6-RPB1 interface in yeast RNAP II, confirms the structural relationship and suggests a "latching" mechanism for the role of ω and RPB6 in promoting RNAP assembly.

Original language	English (US)
Pages (from-to)	892-897
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	98
Issue number	3
DOIs	https://doi.org/10.1073/pnas.98.3.892
State	Published - Jan 30 2001

All Science Journal Classification (ASJC) codes

General

Keywords

RP81 subunit
Transcription
β′ subunit

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Minakhin, L., Bhagat, S., Brunning, A., Campbell, E. A., Darst, S. A., Ebright, R. H., & Severinov, K. (2001). Bacterial RNA polymerase subunit ω and eukaryotic polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly. Proceedings of the National Academy of Sciences of the United States of America, 98(3), 892-897. https://doi.org/10.1073/pnas.98.3.892

Minakhin, Leonid ; Bhagat, Sechal ; Brunning, Adrian ; Campbell, Elizabeth A. ; Darst, Seth A. ; Ebright, Richard H. ; Severinov, Konstantin. / Bacterial RNA polymerase subunit ω and eukaryotic polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 3. pp. 892-897.

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title = "Bacterial RNA polymerase subunit ω and eukaryotic polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly",

abstract = "Bacterial DNA-dependent RNA polymerase (RNAP) has subunit composition β′βαIαII. The role of ω has been unclear. We show that is homologous in sequence and structure to RPB6, an essential subunit shared in eukaryotic RNAP I, II, and III. In Escherichia coli, overproduction of ω suppresses the assembly defect caused by substitution of residue 1362 of the largest subunit of RNAP,β′. In yeast, overproduction of RPB6 suppresses the assembly defect caused by the equivalent substitution in the largest subunit of RNAP II, RPB1. High-resolution structural analysis of the ω-β′ interface in bacterial RNAP, and comparison with the RPB6-RPB1 interface in yeast RNAP II, confirms the structural relationship and suggests a {"}latching{"} mechanism for the role of ω and RPB6 in promoting RNAP assembly.",

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Minakhin, L, Bhagat, S, Brunning, A, Campbell, EA, Darst, SA, Ebright, RH & Severinov, K 2001, 'Bacterial RNA polymerase subunit ω and eukaryotic polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly', Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 3, pp. 892-897. https://doi.org/10.1073/pnas.98.3.892

Bacterial RNA polymerase subunit ω and eukaryotic polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly. / Minakhin, Leonid; Bhagat, Sechal; Brunning, Adrian; Campbell, Elizabeth A.; Darst, Seth A.; Ebright, Richard H.; Severinov, Konstantin.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 3, 30.01.2001, p. 892-897.

Research output: Contribution to journal › Article › peer-review

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AU - Campbell, Elizabeth A.

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AB - Bacterial DNA-dependent RNA polymerase (RNAP) has subunit composition β′βαIαII. The role of ω has been unclear. We show that is homologous in sequence and structure to RPB6, an essential subunit shared in eukaryotic RNAP I, II, and III. In Escherichia coli, overproduction of ω suppresses the assembly defect caused by substitution of residue 1362 of the largest subunit of RNAP,β′. In yeast, overproduction of RPB6 suppresses the assembly defect caused by the equivalent substitution in the largest subunit of RNAP II, RPB1. High-resolution structural analysis of the ω-β′ interface in bacterial RNAP, and comparison with the RPB6-RPB1 interface in yeast RNAP II, confirms the structural relationship and suggests a "latching" mechanism for the role of ω and RPB6 in promoting RNAP assembly.

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Minakhin L, Bhagat S, Brunning A, Campbell EA, Darst SA, Ebright RH et al. Bacterial RNA polymerase subunit ω and eukaryotic polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly. Proceedings of the National Academy of Sciences of the United States of America. 2001 Jan 30;98(3):892-897. https://doi.org/10.1073/pnas.98.3.892