A species of low molecular weight RNA (M3) which is produced in bacteriophage φ80-infected Escherichia coli has been isolated and characterized. M3 appears immediately after infection and its synthesis continues until lysis. This RNA is unstable; its mean half-life is 13.5 min. The structural gene for M3 is localized on the φ80 genome to the right of the exonuclease locus and to the left of gene Q. The complete nucleotide sequence of M3 has been determined. It is 62 nucleotides in length. The 5′ end begins with pppGp and the 3′ terminus is C-U-U-U-U-U-A-AOH. The sequence and sensitivity to ribonuclease digestion suggests a highly double-stranded structure for M3.
All Science Journal Classification (ASJC) codes
- Molecular Biology