Bacteriophage φ80-induced low molecular weight RNA

George Pieczenik; B. G. Barrell; Malcolm L. Gefter

doi:10.1016/0003-9861(72)90203-2

Bacteriophage φ80-induced low molecular weight RNA

George Pieczenik, B. G. Barrell, Malcolm L. Gefter

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

A species of low molecular weight RNA (M₃) which is produced in bacteriophage φ80-infected Escherichia coli has been isolated and characterized. M₃ appears immediately after infection and its synthesis continues until lysis. This RNA is unstable; its mean half-life is 13.5 min. The structural gene for M₃ is localized on the φ80 genome to the right of the exonuclease locus and to the left of gene Q. The complete nucleotide sequence of M₃ has been determined. It is 62 nucleotides in length. The 5′ end begins with pppGp and the 3′ terminus is C-U-U-U-U-U-A-A_OH. The sequence and sensitivity to ribonuclease digestion suggests a highly double-stranded structure for M₃.

Original language	English (US)
Pages (from-to)	152-165
Number of pages	14
Journal	Archives of Biochemistry and Biophysics
Volume	152
Issue number	1
DOIs	https://doi.org/10.1016/0003-9861(72)90203-2
State	Published - Sep 1972
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology

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10.1016/0003-9861(72)90203-2

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title = "Bacteriophage φ80-induced low molecular weight RNA",

abstract = "A species of low molecular weight RNA (M3) which is produced in bacteriophage φ80-infected Escherichia coli has been isolated and characterized. M3 appears immediately after infection and its synthesis continues until lysis. This RNA is unstable; its mean half-life is 13.5 min. The structural gene for M3 is localized on the φ80 genome to the right of the exonuclease locus and to the left of gene Q. The complete nucleotide sequence of M3 has been determined. It is 62 nucleotides in length. The 5′ end begins with pppGp and the 3′ terminus is C-U-U-U-U-U-A-AOH. The sequence and sensitivity to ribonuclease digestion suggests a highly double-stranded structure for M3.",

author = "George Pieczenik and Barrell, {B. G.} and Gefter, {Malcolm L.}",

note = "Funding Information: The authors thank T. V. Smith for expert technical assistance and Dr. F. Banger for helpful discussions. This work was supported by Grant E-561 from the American Cancer Society and Project No. 273 from the Jane Coffin Childs Memorial Fund for Medical Research to M. G.",

year = "1972",

month = sep,

doi = "10.1016/0003-9861(72)90203-2",

language = "English (US)",

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journal = "Archives of Biochemistry and Biophysics",

issn = "0003-9861",

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T1 - Bacteriophage φ80-induced low molecular weight RNA

AU - Pieczenik, George

AU - Barrell, B. G.

AU - Gefter, Malcolm L.

N1 - Funding Information: The authors thank T. V. Smith for expert technical assistance and Dr. F. Banger for helpful discussions. This work was supported by Grant E-561 from the American Cancer Society and Project No. 273 from the Jane Coffin Childs Memorial Fund for Medical Research to M. G.

PY - 1972/9

Y1 - 1972/9

N2 - A species of low molecular weight RNA (M3) which is produced in bacteriophage φ80-infected Escherichia coli has been isolated and characterized. M3 appears immediately after infection and its synthesis continues until lysis. This RNA is unstable; its mean half-life is 13.5 min. The structural gene for M3 is localized on the φ80 genome to the right of the exonuclease locus and to the left of gene Q. The complete nucleotide sequence of M3 has been determined. It is 62 nucleotides in length. The 5′ end begins with pppGp and the 3′ terminus is C-U-U-U-U-U-A-AOH. The sequence and sensitivity to ribonuclease digestion suggests a highly double-stranded structure for M3.

AB - A species of low molecular weight RNA (M3) which is produced in bacteriophage φ80-infected Escherichia coli has been isolated and characterized. M3 appears immediately after infection and its synthesis continues until lysis. This RNA is unstable; its mean half-life is 13.5 min. The structural gene for M3 is localized on the φ80 genome to the right of the exonuclease locus and to the left of gene Q. The complete nucleotide sequence of M3 has been determined. It is 62 nucleotides in length. The 5′ end begins with pppGp and the 3′ terminus is C-U-U-U-U-U-A-AOH. The sequence and sensitivity to ribonuclease digestion suggests a highly double-stranded structure for M3.

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Bacteriophage φ80-induced low molecular weight RNA

Abstract

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