Bacteriophage T7 morphogenesis and gene 10 frameshifting in Escherichia coli showing different degrees of ribosomal fidelity

John Sipley, John Dunn, Emanuel Goldman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Bacteriophage T7 infection has been studied in Escherichia coli strains showing both increased and decreased ribosome fidelity and in the presence of streptomycin, which stimulates translational misreading, in an effort to determine effects on the apparent programmed translational frameshift that occurs during synthesis of the gene 10 capsid protein. Quantitation of the protein bands from SDS-PAGE failed to detect any significant effects on the amounts of the shifted 10B protein relative to the in-frame 10A protein under all fidelity conditions tested. However, any changes in fidelity conditions led to inhibition of phage morphogenesis in single-step growth experiments, which could not be accounted for by reduced amounts of phage protein synthesis, nor, at least in the case of decreased accuracy, by reduced amounts of phage DNA synthesis. Reduction in phage DNA synthesis did appear to account for a substantial proportion of the reduction in phage yield seen under conditions of increased accuracy. Similar effects of varying ribosomal fidelity on growth were also seen with phage T3, and to a lesser extent with phage T4. The absence of change in the high-frequency T7 gene 10 frameshift differs from earlier reports that ribosomal fidelity affects low-frequency frameshift errors.

Original languageEnglish (US)
Pages (from-to)376-384
Number of pages9
JournalMgg Molecular & General Genetics
Volume230
Issue number3
DOIs
StatePublished - Dec 1 1991
Externally publishedYes

Fingerprint

Bacteriophage T7
Morphogenesis
Bacteriophages
Escherichia coli
Genes
Proteins
Bacteriophage T3
Bacteriophage T4
DNA
Capsid Proteins
Streptomycin
Growth
Ribosomes
Gene Frequency
Polyacrylamide Gel Electrophoresis
Infection

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • Frameshifting
  • Phages T7, T3, and T4
  • Ribosomal fidelity
  • Streptomycin
  • Translational accuracy

Cite this

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title = "Bacteriophage T7 morphogenesis and gene 10 frameshifting in Escherichia coli showing different degrees of ribosomal fidelity",
abstract = "Bacteriophage T7 infection has been studied in Escherichia coli strains showing both increased and decreased ribosome fidelity and in the presence of streptomycin, which stimulates translational misreading, in an effort to determine effects on the apparent programmed translational frameshift that occurs during synthesis of the gene 10 capsid protein. Quantitation of the protein bands from SDS-PAGE failed to detect any significant effects on the amounts of the shifted 10B protein relative to the in-frame 10A protein under all fidelity conditions tested. However, any changes in fidelity conditions led to inhibition of phage morphogenesis in single-step growth experiments, which could not be accounted for by reduced amounts of phage protein synthesis, nor, at least in the case of decreased accuracy, by reduced amounts of phage DNA synthesis. Reduction in phage DNA synthesis did appear to account for a substantial proportion of the reduction in phage yield seen under conditions of increased accuracy. Similar effects of varying ribosomal fidelity on growth were also seen with phage T3, and to a lesser extent with phage T4. The absence of change in the high-frequency T7 gene 10 frameshift differs from earlier reports that ribosomal fidelity affects low-frequency frameshift errors.",
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Bacteriophage T7 morphogenesis and gene 10 frameshifting in Escherichia coli showing different degrees of ribosomal fidelity. / Sipley, John; Dunn, John; Goldman, Emanuel.

In: Mgg Molecular & General Genetics, Vol. 230, No. 3, 01.12.1991, p. 376-384.

Research output: Contribution to journalArticle

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